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Identification of genes whose expressions are affected by ptc + dpp double RNAi in spider embryos

ABSTRACT: Identification of genes whose expressions are affected by ptc + dpp double RNAi in spider embryos

PROVIDER: PRJNA446002 | ENA |

REPOSITORIES: ENA

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Identification of genes whose expression is affected by ptc + dpp double RNAi in spider embryos

Project description:In the spider Parasteatoda tepidariorum, Hedgehog (Hh) and Decapentaplegic (Dpp) signaling play key roles in the formation of the two major embryonic axes (Akiyama-Oda and Oda, 2010, Development 137, 1263-1273; Akiyama-Oda and Oda, 2006, Development 133, 2347-2357). Patched (Ptc), a receptor for Hh,functions as a negative regulator of Hh signaling. Double parental RNA interference (pRNAi) against spider homologs of ptc and dpp, Pt-ptc and Pt-dpp, respectively, results in severe defects in axis formation. In this microarray experiment, we attempted to identify candidate genes whose expressions are regulated by Hh and Dpp signaling during axis formation.

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Identification of genes whose expressions are affected by hedgehog RNAi in spider embryos

Project description:In the spider Achaearanea tepidariorum, Hedgehog (Hh) signaling plays a key role in the formation of the two major embryonic axes. Analyses of expression patterns of the spider hh homolog, At-hh, suggested that Hh signaling might be involved in the subsequent segmentation process also. In this microarray experiment, we attempted to identify candidate genes whose expressions are regulated by Hh signaling during early phases of spider segmentation. The objective of this experiment was a screen for genes whose expressions might be affected by parental RNA interference (pRNAi) against At-hh in spider embryos. Oligonucleotide probes were designed based on accumulated A. tepidariorum EST sequences and embedded in custom microarrays (12K x2). Total RNA was extracted from late stage 5 embryos derived from a mated female before and after injection of dsRNA targeted for At-hh. Gene expression levels were compared between the untreated (normal) and the At-hh pRNAi-treated samples. Probes for At-hh and a homolog of Drosophila orthodenticle, At-otd, served as positive controls to validate this experiment.

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Identification of genes whose expression is affected by Delta RNAi in spider embryos

Project description:In the spider Parasteatoda tepidariorum embryo, Delta-Notch signaling plays a key role in early development of the opisthosomal region by regulating the balance between the caudal ectoderm and mesoderm fates (Oda et al., 2007, Development 134, 2195-2205). In this microarray experiment, we attempted to identify candidate genes whose expressions are regulated by Delta-Notch signaling during early phases of the caudal development.

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Identification of genes whose expressions are affected by Delta RNAi in spider embryos

Project description:Identification of genes whose expressions are affected by Delta RNAi in spider embryos

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Identification of genes whose expression is affected by fuchi nashi pRNAi in early embryos of the spider Parasteatoda tepidariorum

Project description:We identified a GATA-like gene named fuchi nashi (fuchi) as a gene required for establishing a germ disc in the early embryo of the spider Parasteatoda tepidariorum. fuchi transcript is initially expressed in cells at both polar regions of the embryo and later in endodermal and extraembryonic cells. To genome-widely identify genes whose expressions are regulated by fuchi, we conducted RNA-sequencing of whole embryos at three different early stages (stages 2 and 3 and early stage 5) that were untreated (control) or treated for fuchi parental RNA interference (pRNAi).

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Project description:Hedgehog signaling plays pivotal roles in formation of the embryonic anterior-posterior axis in the spider Parasteatoda tepidariorum. In this work, using the combination of RNA-sequencing and parental RNA interference (pRNAi), we genome-widely identified genes that transcriptionally responded to altered states of Hedgehog signaling in spider embryos, which were caused by Pt-hedgehog (Pt-hh) pRNAi and Pt-patched (Pt-ptc) pRNAi. The identified genes were classified into four classes (Classes I to IV) based on the positive/negative responses to the Pt-hh pRNAi and Pt-ptc pRNAi states. The Class II genes were negatively regulated by Hh signaling (positive response to Pt-hh pRNAi and negative response to Pt-ptc pRNAi). Among the Class II genes, we identified Pt-msx1 as a key segmentation gene in the spider embryo. Moreover, we conducted a transcriptomic analysis of Pt-msx1 pRNAi embryos and identified additional genes whose expression showed patterns associated with segmentation.

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Identification of genes whose expression is affected by fuchi nashi pRNAi in early embryos of the spider Parasteatoda tepidariorum

Project description:Identification of genes whose expression is affected by fuchi nashi pRNAi in early embryos of the spider Parasteatoda tepidariorum

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Identification of Dpp target genes during the early embryo development

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