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Transcriptome analysis of rhesus monkey germinal vesicle, failed-to-mature oocytes and MII oocytes

ABSTRACT: Transcriptome analysis of rhesus monkey germinal vesicle, failed-to-mature oocytes and MII oocytes

PROVIDER: PRJNA448148 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6919690.fastq.gz	Fastqsanger.gz
	SRR6919700.fastq.gz	Fastqsanger.gz
	SRR6919691.fastq.gz	Fastqsanger.gz
	SRR6919701.fastq.gz	Fastqsanger.gz
	SRR6919692.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of rhesus monkey germinal vesicle and failed-to-mature oocytes

Project description:Oocyte maturation defects are one contributing factor in unexplained female infertility. Failure of some oocytes to undergo germinal vesicle breakdown or progress to second meiotic metaphase in response to an ovulatory stimulus can limit the number of high quality oocytes available for assisted reproduction. Understanding the molecular mechanisms that underlie oocyte maturation failure may lead to novel methods for increasing the number of high quality oocytes available for assisted reproduction, as well as providing new insight into the basic biology of oocyte maturation. Using RNA sequencing, we compared the transcriptome of rhesus monkey oocytes that failed to mature (FTM) in response to an ovulatory stimulus in vivo to normal germinal vesicle and metaphase II stage oocytes.

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Transcriptome analysis of rhesus monkey MII oocytes

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Transcriptome analysis of rhesus monkey germinal vesicle and failed-to-mature oocytes

Project description:Transcriptome analysis of rhesus monkey germinal vesicle and failed-to-mature oocytes

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Transcriptome analysis of rhesus monkey MII oocytes

Project description:Transcriptome analysis of rhesus monkey MII oocytes

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Contrast of poly-ribosomal mRNA in germina vesicle (GV) and metaphase II (MII) bovine oocytes.

Project description:Poly-ribosomal mRNA from pools of 75 bovine oocytes at the germinal vesicle(GV) and metaphase II(MII) stages were isolated by sucrose fractionation then compared through microarray hybridization. 3 biological replicates from germinal vesicle(GV) and metaphase II(MII) bovine oocytes were hybridized against each other while alternating dyes. One additional technical replicate (dye-swap of one of the previous hybridizations) was added to balance dye usage. The normalized log2 ratio (GV/MII) data is provided in the 'normalized_data.txt' file. Please note that data were scanned/extracted using a different Agilent reference file, and thus the feature extraction number for a given probe (in both normalized and raw data files) is different from that in the official Agilent-041017 platform (GPL18537). The reference file used for this study is provided as a Series supplementary file (041017_D_DNAFront_BCBottom_20120517.txt).

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Contrast of poly-ribosomal mRNA in germina vesicle (GV) and metaphase II (MII) bovine oocytes.

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Transcriptome analysis of rhesus monkey germinal vesicle, failed-to-mature oocytes and MII oocytes

Project description:This SuperSeries is composed of the SubSeries listed below.

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Transcriptome analysis of rhesus monkey MII oocytes and embryos

Project description:Transcriptome analysis of rhesus monkey MII oocytes and embryos

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Microarray data of prepubertal, peripubertal, and adult oocytes, and from GV and MII oocytes matured in vivo or in vitro

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