Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

10x analysis of wild-type (TLAB) and MZoep zebrafish embryos at 6-somite stage

ABSTRACT: 10x analysis of wild-type (TLAB) and MZoep zebrafish embryos at 6-somite stage

PROVIDER: PRJNA449093 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

10x analysis of wild-type (TLAB) and MZoep zebrafish embryos at 6-somite stage

Project description:Wild-type and MZoep zebrafish embryos were mechanically dissociated and profiled using 10x Genomics pipeline.

2018-04-27 | GSE112824 | GEO

Drop-seq analysis of wild-type (TLAB) zebrafish embryos from high to 6-somite stage (12 timepoints)

Project description:Drop-seq analysis of wild-type (TLAB) zebrafish embryos from high to 6-somite stage (12 timepoints)

| PRJNA417290 | ENA

Integrin a5, aV, and aVb3 in zebrafish embryos at the 12 somite stage

Project description:Integrins are a major class of heterodimeric adhesion receptors composed of an a and b subunit that mediate cell adhesion to the extracellular matrix (ECM). The extracellular matrix protein fibronectin is important for early vertebrate development, and Integrin a5b1 and aVb3 are the two primary fibronectin receptors. To better define the integrin – ECM protein network at 10-13 somite stage of zebrafish development, we performed co-immunoprecipitation and Mass Spectrometry (MS) based proteomics using FLAG-tagged Integrin a5, aV, and aVb3 expressed in maternal zygotic a5 mutant (MZa5-/-) embryos. We found that Integrin a5b1 and aVb1 are the functional fibronectin receptors, whereas Integrin aVb3 displayed low affinity to both fibronectins (Fn1a and Fn1b). In addition, basement membrane ligands Laminins (lama1, lamb1a, lamc1) are roughly equal in all three datasets while Thrombospondins (thbs3b, thbs4b) and cartilage oligomeric matrix protein (comp/thbs5) are found exclusively in the aV dataset. Our results suggest a diverse role of aV class integrins in ECM protein recruitment.

2021-09-10 | PXD024665 | Pride

ATAC-seq profile of zebrafish periderm at 4-somite stage

Project description:In order to profile periderm-specific nucleosome free regions of zebrafish periderm cells at 4-somite stage, we isolated the equal number of GFP-positive cells and GFP-negative cells from Tg(krt4:GFP) embryos and perform ATAC-seq. This study revealed the landscape of zebrafish periderm-specific nucleosome free regions at 4-somite stage.

2020-01-13 | GSE140240 | GEO

mRNA profile of zebrafish periderm at 4-somite stage

Project description:In order to compare the transcriptome and tissue-specific nucleosome free regions of zebrafish periderm cells at 4-somite stage, we isolated the RNA from sorted GFP-positive cells of Tg(krt4:GFP) embryos and perform low-input RNA-seq. This study revealed periderm-specific transcriptome at 4-somite stage. Moreover, comparing with ATAC-seq from the same tissue, we confirmed the correlation between gene expression and nearby chromatin accessibility.

2020-01-13 | GSE139808 | GEO

RNAseq from 29 somite stage FNP

Project description:RNAseq from somite-matched frontonasal prominence (FNP) from E10.5 embryos at the 29-somite developmental stage

2021-05-08 | GSE174090 | GEO

Drop-seq analysis of wild-type (TLAB) zebrafish embryos from high to 6-somite stage (12 timepoints)

Project description:Wild-type zebrafish embryos were mechanically dissociated and profiled using Drop-seq

2018-04-27 | GSE106474 | GEO

Sequencing of smallRNAs of fish embryos in somite stage

Project description:Sequencing of smallRNAs of fish embryos in somite stage

| PRJNA321022 | ENA

High-throughput sequencing of small RNAs in chicken somite embryos

Project description:High-throughput sequencing of small RNAs in chicken somite embryos