Project description:Proanthocyanidins (PAs) in sainfoin (Onobrychis viciifolia Scop.) are of interest to ameliorate the sustainability of livestock production. However, sainfoin forage yield and PA concentrations, as well as their composition, require optimization. Individual plants of 27 sainfoin accessions from four continents were analyzed with LC-ESI-QqQ-MS/MS for PA concentrations and simple phenolic compounds. Large variability existed in PA concentrations (23.0-47.5 mg g(-1) leaf dry matter (DM)), share of prodelphinidins (79-96%), and mean degree of polymerization (11-14) among, but also within, accessions. PAs were mainly located in leaves (26.8 mg g(-1) DM), whereas stems had less PAs (7.8 mg g(-1) DM). Overall, high-yielding plants had lower PA leaf concentrations (R(2) = 0.16, P < 0.001) and fewer leaves (R(2) = 0.66, P < 0.001). However, the results show that these two trade-offs between yield and bioactive PAs can be overcome.
Project description:Sainfoin (Onobrychis viciifolia) is one of the dominant legume forages distributed in Northern China. In our study, we assembled and annotated the structure of the complete chloroplast genome of sainfoin. The length of the circular genome is 122,102 bp. It contains 115 genes, including 79 protein-coding genes (68.7%), 31 tRNA genes (26.96%) and 5 rRNA genes (4.35%). The GC content of the total chloroplast genome of sainfoin is 34.58%. We construct the phylogenetic relationships between the chloroplast genome of sainfoin and the other 16 species by the Maximum likelihood (ML), and found sainfoin is most closely related to Hedysarum petrovii and Hedysarum taipeicum.