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Transcriptome of primary and metastatic human melanoma with silenced STAT3

ABSTRACT: Transcriptome of primary and metastatic human melanoma with silenced STAT3

PROVIDER: PRJNA476058 | ENA |

REPOSITORIES: ENA

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Transcriptome of primary and metastatic human melanoma with silenced STAT3

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EZH2-mediated primary cilium deconstruction drives metastatic melanoma formation

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EZH2-mediated primary cilium deconstruction drives metastatic melanoma formation

Project description:Human melanomas frequently harbor amplifications of EZH2. However, the oncogenic contribution of this methyltransferase to melanoma formation has remained elusive. Taking advantage of murine melanoma models, we now show that EZH2 drives tumorigenesis from benign BrafV600E or NrasQ61K-expressing melanocytes. EZH2 oncogenicity results from silencing of genes relevant for the integrity of the primary cilium, a signaling organelle projecting from the surface of vertebrate cells. Consequently, gain of EZH2 function promotes loss of primary cilia in benign melanocytic lesions. In contrast, blockade of EZH2 activity evokes ciliogenesis and cilia-dependent growth inhibition in malignant melanoma. Finally, we demonstrate that loss of cilia enhances pro-tumorigenic WNT/β-catenin signaling and is itself sufficient to drive metastatic melanoma in benign cells. Thus, primary cilia deconstruction is a key process in EZH2-driven melanomagenesis.

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STAT3 promotes melanoma metastasis by CEBP-induced repression of the MITF pigmentation pathway

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