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Plum pox virus

ABSTRACT: Plum pox virus Genome sequencing and assembly

PROVIDER: PRJNA479658 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7472384_1.fastq.gz	Fastqsanger.gz
	SRR7472384_2.fastq.gz	Fastqsanger.gz
	SRR7472385_1.fastq.gz	Fastqsanger.gz
	SRR7472385_2.fastq.gz	Fastqsanger.gz
	SRR7472386_1.fastq.gz	Fastqsanger.gz

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Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection

2008-11-04 | GSE11217 | GEO

Transcription profiling of Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection

Project description:Virus infection induces activation and suppression of global gene expression in the host. Profiling gene expression changes in the host may provide insights into the molecular mechanisms that underlie host physiological and phenotypic responses to virus infection. In this study, the Arabidopsis Affymetrix ATH1 whole genome array was used to assess global gene expression changes in Arabidopsis thaliana plants infected with Plum pox virus (PPV). To identify early genes in response to PPV infection, an Arabidopsis synchronized single-cell transformation system was developed. Arabidopsis protoplasts were transfected with a PPV infectious clone, PPV-SK68 and global gene expression changes in the transfected protoplasts were profiled. Experiment Overall Design: For PPV infection in Arabdiopsis leaves, eight independent hybridizations were performed using total RNA isolated from three independent biological replicates of the virus-infected or mock-inoculated control samples. Experiment Overall Design: For PPV infection in Arabidopsis protoplasts, 24 gene chips in total were used to hybridize with RNA isolated from protoplasts transfected with PPV infectious clone and PPV deletion mutant.

2008-11-07 | E-GEOD-11217 | biostudies-arrayexpress

Transcriptomic analysis of Nicotiana benthamiana plants infected with a plum pox virus (PPV) deletion mutant (P1Pro) that lacks the self-cleavage inhibitory domain of the P1 leader protease

Project description:Transcriptomes of wild-type Nicotiana benthamiana plants inoculated with plum pox virus (PPV) or the P1Pro clone, a PPV deletion mutant that lacks the self-cleavage inhibitory domain of the P1 leader protease; in addition, N. benthamiana nahG-expressing plants inoculated with P1Pro were analyzed to identify genes whose expression is altered by P1Pro infections but does not depend on salicylic acid signaling.

2020-07-30 | GSE146746 | GEO

Common and strain-specific post-translational modifications of the potyvirus Plum pox virus coat protein in different hosts

Project description:Phosphorylation and O-GlcNAcylation are widespread post-translational modifications (PTMs) often sharing protein targets. Numerous studies have reported phosphorylation of plant virus proteins. In plants, research on O-GlcNAcylation lags behind regarding other eukaryotes and information about O-GlcNAcylated plant viral proteins is extremely scarce. The potyvirus Plum pox virus (PPV) causes sharka disease in Prunus trees, and also infects a wide range of experimental hosts. Capsid protein (CP) from virions of the PPV-R isolate purified from herbaceous plants can be extensively modified by O-GlcNAcylation and phosphorylation. In this study, a combination of proteomics and biochemical approaches has been employed to broaden knowledge of PPV CP PTMs. CP proved to be modified regardless it is assembled or not in mature particles. PTMs of CP occur in the natural host Prunus persica, similar to what happens in herbaceous plants. Additionally, we observe O-GlcNAcylation and phosphorylation are general features of different PPV strains, suggesting that roles of these modifications are part of overall strategies deployed during plant-virus interactions. Interestingly, phosphorylation at a casein kinase II motif conserved among potyviral CPs exhibits strain specificity in PPV; however, it does not display the critical role attributed to same modification in the CP of another potyvirus, Potato virus A.

2020-05-27 | PXD017780 | Pride

Phloem and non-phloem associated translatomes during plum pox virus infection in Prunus domestica L.

Project description:In this study we used a translating ribosome affinity purification strategy to identify phloem and non-phloem associated translatomes in Prunus domesitca L during PPV infection. Three different promoter:His6FLAG-RPL18 lines were used. These included two phloem specific promoters (pSUC2 and pSULTR2;2) as well as the more ubiquitously expressed cauliflower mosaic virus 35S promoter (p35S). Immunopurification of ribosome-mRNA complexes was accomplished by the method described in Reynoso et al. (Plant Functional Genomics: Methods and Protocols, 185-207; 2015). The dataset includes samples from plum leaves taken at 2, 4, 6, and 12 weeks post cold induced dormancy.

2019-07-30 | GSE131832 | GEO

Phloem and non-phloem associated translatomes during plum pox virus infection in Prunus domestica L. II

2020-05-15 | GSE150606 | GEO

Involvement of different plant viruses in the activation of RNA silencing-related genes and the defensive response against Plum pox virus of ‘GF305’ peach (Prunus persica) grafted with ‘Garrigues’ almond (P. dulcis)

Project description:Plum pox virus (PPV) causes the serious sharka disease in Prunus trees. Peach [P. persica (L.) Batsch] trees are severely affected by PPV and no definitive source of genetic resistance has been identified at this moment. Previous results showed, however, that PPV-resistant ‘Garrigues’ almond [P. dulcis (Mill.) D.A. Webb] was able to transfer its resistance to ‘GF305’ peach through grafting, preventing these trees from PPV infection and reducing symptomatology and viral load in PPV-infected plants. A recent study tried to identify genes responsible for this effect by studying mRNA expression through RNAseq data in peach and almond plants, before and after grafting, and before and after PPV infection. In this work, we used the same peach and almond samples, but focused the high-throughput analyses on small RNAs (sRNAs) expression. We studied massive sequencing data and found an interesting pattern of sRNAs overexpression linked to antiviral defense genes that suggested activation of these genes followed by downregulation to basal levels. We also discovered that ‘Garrigues’ almond plants were infected by different plant viruses that were transferred to peach plants. The large amounts of viral sRNAs found in grafted peaches indicated a strong RNA silencing antiviral response and led us to postulate that these plant viruses could be collaborating by cross-protection in the observed ‘Garrigues’ effect.

2022-11-02 | GSE188935 | GEO

Phloem and non-phloem associated translatomes during plum pox virus infection in Prunus domestica L.

Project description:Phloem and non-phloem associated translatomes during plum pox virus infection in Prunus domestica L.

| PRJNA545091 | ENA

Phloem and non-phloem associated translatomes during plum pox virus infection in Prunus domestica L. II

Project description:Phloem and non-phloem associated translatomes during plum pox virus infection in Prunus domestica L. II

| PRJNA632894 | ENA

MiSeq of viral-derived RT-PCR amplicons from plants infected with different versions of plum pox virus

Project description:MiSeq of viral-derived RT-PCR amplicons from plants infected with different versions of plum pox virus

| PRJEB34682 | ENA