Ontology highlight
ABSTRACT:
PROVIDER: PRJNA480128 | ENA |
REPOSITORIES: ENA
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Project description:Salt marshes provide many key ecosystem services that have tremendous ecological and economic value. One critical service is the removal of fixed nitrogen from coastal waters, which limits the negative effects of eutrophication resulting from increased nutrient supply. Nutrient enrichment of salt marsh sediments results in higher rates of nitrogen cycling and, commonly, a concurrent increase in the flux of nitrous oxide, an important greenhouse gas. Little is known, however, regarding controls on the microbial communities that contribute to nitrous oxide fluxes in marsh sediments. To address this disconnect, we generated microbial community profiles as well as directly assayed nitrogen cycling genes that encode the enzymes responsible for overall nitrous oxide flux from salt marsh sediments. We hypothesized that communities of microbes responsible for nitrogen transformations will be structured by nitrogen availability. Taxa that respond positively to high nitrogen inputs may be responsible for the elevated rates of nitrogen cycling processes measured in fertilized sediments. Our data show that, with the exception of ammonia-oxidizing archaea, the community composition of organisms responsible for production and consumption of nitrous oxide was altered under nutrient enrichment. These results suggest that elevated rates of nitrous oxide production and consumption are the result of changes in community structure, not simply changes in microbial activity.
2018-01-13 | GSE108888 | GEO
Project description:Strophostyles helvola is a close relative to common bean (Phaseolus vulgaris) and inhabits both coastal and non-coastal regions in North America. However, the mechanism of saline adaptation in S. helvola remains unclear. A transcriptome profiling would facilitate dissecting the underlying molecular mechanisms in salinity-adapted S. helvola. In this study, we reported the RNAseq analyses of two genotypes (a salt-tolerant beach genotype and a salt-sensitive inland genotype) of S. helvola stressed with salt. S. helvola plants were grown in pots and treated with half lethal-guided dose of NaCl solution for 3h, 24h, and 7d. The plants supplied with the same amount of water were used as controls. The whole roots sampled from the three time points were equally pooled as one biological replicate, and three replicates were used for library construction and transcriptome sequencing on Illumina Hiseq 2500. The comparative analyses of root transcriptomes presented here provides a valuable resource for discovery of genes and networks involved in salt tolerance in S. helvola.
2018-12-06 | GSE121143 | GEO
Project description:Salt marshes sediment microhabitats Metagenome
| PRJNA236470 | ENA
Project description:Salt sensitivity of blood pressure (SSBP) is an independent risk factor for cardiovascular disease. However, the pathogenic mechanisms of SSBP are still uncertain. Thus, ceRNA microarray was applied to identify differentially expressed lncRNAs and mRNAs. The whole blood samples from 10 hypertensives and 10 normotensives were collected by professional nurses in community health centers using EDTA blood tube. The samples were classified according to the salt sensitivity (SS, salt sensitivity; SR, salt resistant) and hypertension (H, hypertensives; N, normotensives). QRT-PCR and cell experiments would be also implemented to validate the reliability of the results.
2019-07-31 | GSE135111 | GEO
Project description:Coastal marshes (Louisiana) Targeted Locus (Loci)
| PRJNA232457 | ENA