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Transcriptomic analysis of Fusobacterium nucleatum-stimulated Human gingival fibroblasts

ABSTRACT: Transcriptomic analysis of Fusobacterium nucleatum-stimulated Human gingival fibroblasts

PROVIDER: PRJNA486466 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7705430.fastq.gz	Fastqsanger.gz
	SRR7705440.fastq.gz	Fastqsanger.gz
	SRR7705450.fastq.gz	Fastqsanger.gz
	SRR7705460.fastq.gz	Fastqsanger.gz
	SRR7705470.fastq.gz	Fastqsanger.gz

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Project description:Neutrophils are known to be stimulated by different periodontal bacteria to produce reactive oxygen species and cytokines. It is inportant to investigate the gene changes made by bacteria of importance, of which, for periodontal disease, fusobaterium nucleatum is one. we used microarrays to investigate gene experssion changes in peripheral blood neutrophils werwhich e stimulated with or with out Fusobacterium Nucleatum (10953). Neutrophils from periodonatlly healthy individuals (n=4) were isolated and stimulated for 3hrs with or without fusobaterium nucleatum (10953). RNA was then extracted from these and pooled before hybridization on Affymetrix microarrays

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