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Analysis of Fusarium verticillioides gene expression in response to nitric oxide (NO)

ABSTRACT: Analysis of Fusarium verticillioides gene expression in response to nitric oxide (NO)

PROVIDER: PRJNA486820 | ENA |

REPOSITORIES: ENA

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Analysis of Fusarium verticillioides gene expression in response to nitric oxide

Project description:Investigation of whole genome gene expression level changes in F. verticillioides M3125 when exposed to 1.5 mM DETA NONOate for 30 minutes. Assessed in reference to unexposed M3125. DETA NONOate is a nitric oxide (NO) donor with a half-life of approximately 56 hours at 27°C, pH 7.4, to liberate 2 moles of NO per mole of parent compound.

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Analysis of Fusarium verticillioides gene expression in response to nitric oxide (NO)

Project description:Investigation of whole transcriptional changes in F. verticillioides wild-type (WT) strain FRC M-3125 and gene deletion strain ΔFVEG_10494-6.4 when treated with and without 1.5 mM diethylenetriamine (DETA) NONOate for 30 min, with three biological replicates each. DETA NONOate is a NO-donor molecule.

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Project description:DNA microarray analysis was employed to investigate the transcriptome response to nitric oxide in Pseudomonas aeruginosa. We focused on the role played by the nitric oxide-response regulators DNR and FhpR and an oxygen-response regulator ANR in the response. The transcriptome profiles of the P. aeruginosa strains before and after exposure to nitric oxide under the microaerobic conditions were analyzed. Wild type, its anr, dnr, and fhpR mutants, and the anr mutant that express dnr were used for the analyses. Pseudomonas aeruginosa wild type (PAO1ut), anr mutant (RManr), dnr mutant (RMdnr), anr mutant that constitutively expresses DNR (RManrEXdnr), and fhpR mutant (PDM2665) were cultivated microaerobically in LB in 1-liter jar fermenter. When optical density at 600 nm reached 0.3, nitric oxide-saturated water was added to the medium (final nitric oxide concentration was 20 micro-M). RNA was isolated from a 10 ml aliquot of the culture prior to the addition of nitric oxide and at 5 min after the addition. The experiment was performed in duplicate independent cultures.

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