Project description:An Infinium microarray platform (GPL28271, HorvathMammalMethylChip40) was used to generate DNA methylation data from blood samples from yellow-bellied marmots (Marmota flaviventris). DNA methylation data from n=159 blood samples. All samples were collected as part of a long-term study of a free-living population of yellow-bellied marmots in the Gunnison National Forest, Colorado (USA), where marmots were captured and blood samples collected biweekly during the their active season (May to August). Genomic DNA was extracted with Qiagen DNeasy blood and tissue kit.
Project description:We evaluated genome wide expression patterns in wild yellow-bellied marmots (Marmota flaviventris) prior to either dispersing from or remaining philopatric to their natal colony site. We identified differential expression of genes involved in lipid metabolism and muscle generation. Further, we found a network of genes involved in extra-cellular immune function and MHC class II pathways that increased in dispersers. Our results provide novel insights into the fundamental molecular changes required for natal dispersal in vertebrates.
