Project description:The intent was to study, from transcriptome analysis, shade and drought responses in Solanum tuberosum (potato). We performed Illumina 50 bp single-end RNA-seq in tissues of control and treated var. Spunta wild-type plants. Drought experiments also included two independent AtBBX21-overexpressing (BBX21-OE) potato lines.

2023-07-12 | E-MTAB-12771 | biostudies-arrayexpress

Solanum tuberosum cultivar:Interspecific potato hybrid MSH/14-112 (P8 x Kufri Jyoti)

Project description:Whole genome sequencing of Interspecific potato hybrid MSH/14-112 (P8 x Kufri Jyoti)

| PRJNA565113 | ENA

mRNA transcription profile of potato (Solanum tuberosum L.) in response to explant cutting

Project description:The explant is likely the most essential parameter of plant tissue culture. The vast majority of tissue culture studies have focused almost exclusively on the use of explants as a tool for organogenesis or propagation, and there is limited information that documents the changes that occur in an explant in response to cutting during explant preparation just prior to culture. To better understand the molecular changes occurring during the wounding process that accompanies the preparation of an explant, this study examined the mRNA transcription profile of potato (Solanum tuberosum L. cv. Desirée) single-node segments. To achieve this, RNA-seq analysis was performed to identify significantly differentially expressed genes (DEGs) that existed between uncut in vitro plantlets and cut explants. DEGs were connected to cellular processes in the extracellular region, nucleus, and plasma membrane, and were associated with biosynthesis, carbohydrate metabolism and catabolism, cellular protein modification, growth and development, and response to stress. These results provide a novel perspective of the changes taking place within explants when they are cut and serve as a valuable basis for the study of explant-related stress in plant tissue culture in other plant species, and may provide an understanding of the success or failure of the tissue culture protocol and subsequent establishment of in vitro cultures.

2019-07-25 | GSE123037 | GEO

Solanum tuberosum

Project description:RNA sequencing of SM/11-120 (PCN resistant) and Kufri Jyoti (susceptible) cultivars.

| PRJNA856845 | ENA

Gene expression study after 3 days of salt exposure in a Solanum tuberosum cultivar called Desirée

Project description:The molecular response to salt exposure was studied in the leaves of an S. tuberosum clone using cDNA microarray. Differentially expressed genes were classified according to their known or predicted function and their expression ratio as compared to the control. The major changes upon a 150 mM NaCl exposure in potato leaves occurred in the photosystem apparatus and Calvin cycle: many transcripts coding for proteins belonging to photosystems I and II and chlorophyll synthesis were repressed, whereas cell rescue and transcription factor-related genes were mostly up-regulated. A strong down-regulation of several functional categories including primary metabolism, detoxication apparatus and signal transduction was also observed.

2011-12-03 | GSE34116 | GEO

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