Project description:To further development of our gene expression approach to biodosimetry, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish expression of semaphorin3F. Human colorectal cancer cell HCT116 was infected with empty control or eGFP-Sh-SEMA3F lentivirus,1468 genes was found altered significantly,709 genes was upregulated and 759 genes was downregulated significantly knock down expression of semaphorin3F induced gene expression in human colorectal cancer cell was measured. Three independent experiments were performed for each experiment

2014-09-16 | E-GEOD-61419 | biostudies-arrayexpress

Development of gene expression signatures for knock down expression of ARL4C

Project description:o further development of our gene expression approach to biodosimetry, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish expression of ARL4C. Human primay GBM cell was transfected with control or eGFP-Sh-ARL4C lentivirus, 588 gene are down-regulated and 366 gene are down-regulated.

2019-06-04 | GSE121253 | GEO

CRISPR/Cas9 knock down line

Project description:CRISPR/Cas9 knock down line

| PRJNA521684 | ENA

circRNA expression in METTL3 and YTHDC1 KNOCK-DOWN conditions

Project description:circRNA expression in METTL3 and YTHDC1 KNOCK-DOWN conditions

| PRJNA578279 | ENA

Expression data from Merm1/Wbscr22 knock-down tumor cells

Project description:Expression data from Merm1/Wbscr22 knock-down tumor cells

| PRJNA127485 | ENA

Expression data from knock-down ES stable cell line

Project description:In order to identify the effects of the knock-down of the gene of interest on the mouse ES transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the knock-down cell line. Transcriptome analysis of the knock-down transgenic mouse ES cell line. The knock-down cell line (shE13) was generated by stably expressing a specific short-hairpin RNA against E13 sequence thus knocking-down E13 expression in parental mouse ES cell line E14Tg2a.4 (E14, Hooper M et al., 1987). The specific mouse gene knocked down in the ES cell line is E130012A19Rik.

2012-12-13 | GSE31165 | GEO

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