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Dissection of Metabolic Reprogramming in Polycystic Kidney Disease Reveals Coordinated Rewiring of Bionergetic Pathways

ABSTRACT: Dissection of Metabolic Reprogramming in Polycystic Kidney Disease Reveals Coordinated Rewiring of Bionergetic Pathways

PROVIDER: PRJNA497797 | ENA |

REPOSITORIES: ENA

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Dissection of Metabolic Reprogramming in Polycystic Kidney Disease Reveals Coordinated Rewiring of Bionergetic Pathways

Project description:Analysis of genome-wide transcriptional changes in cystic kidneys in the hypomorphic Pkd1V/V mouse model at postnatal day 10. The hypothesis tested in the present study was that metabolism is reprogrammed in polycystic kidney disease (PKD) in this model. Results provide important information of metabolic changes that affect glycolysis, mitochondrial metabolism, and fatty acid synthesis in the pathogenesis of PKD.

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Dissection of metabolic reprogramming in polycystic kidney disease reveals coordinated rewiring of bioenergetic pathways

Project description:Autosomal Dominant Polycystic Kidney Disease (ADPKD) is a genetic disorder caused by loss-of-function mutations in PKD1 or PKD2. Increased glycolysis is a prominent feature of the disease, but how it impacts on other metabolic pathways is unknown. Here, we present an analysis of mouse Pkd1 mutant cells and kidneys to investigate the metabolic reprogramming of this pathology. We show that loss of Pkd1 leads to profound metabolic changes that affect glycolysis, mitochondrial metabolism, and fatty acid synthesis (FAS). We find that Pkd1-mutant cells preferentially use glutamine to fuel the TCA cycle and to sustain FAS. Interfering with either glutamine uptake or FAS retards cell growth and survival. We also find that glutamine is diverted to asparagine via asparagine synthetase (ASNS). Transcriptional profiling of PKD1-mutant human kidneys confirmed these alterations. We find that silencing of Asns is lethal in Pkd1-mutant cells when combined with glucose deprivation, suggesting therapeutic approaches for ADPKD.

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Polycystic Kidney Disease Progression Expression Analysis

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Expression data from renal cysts of autosomal dominant polycystic kidney disease (ADPKD) patients

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Expression data from renal cysts of autosomal dominant polycystic kidney disease (ADPKD) patients

Project description:To elucidate the molecular pathways that modulate renal cyst growth in autosomal dominant polycystic kidney disease (ADPKD) Keywords: Disease state analysis

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