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Preparing for the first breath at single cell level [single cell Drop-seq]

ABSTRACT: Preparing for the first breath at single cell level [single cell Drop-seq]

PROVIDER: PRJNA504643 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8176396_1.fastq.gz	Fastqsanger.gz
	SRR8176396_2.fastq.gz	Fastqsanger.gz
	SRR8176397_1.fastq.gz	Fastqsanger.gz
	SRR8176397_2.fastq.gz	Fastqsanger.gz
	SRR8176398_1.fastq.gz	Fastqsanger.gz

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Preparing for the first breath at single cell level

Project description:Preparing for the first breath at single cell level

| PRJNA504641 | ENA

Preparing for the first breath at single cell level [single cell Drop-seq]

Project description:The respiratory system undergoes remarkable structural, biochemical, and functional changes necessary for adaptation to air breathing at birth. To identify dynamic changes in gene expression in the diverse pulmonary cells at birth, we performed Drop-seq based massive parallel single-cell RNA sequencing. An iterative cell type identification strategy was used to unbiasedly identify the heterogeneity of murine pulmonary cell types on postnatal day 1. Distinct populations of epithelial, endothelial, mesenchymal, and immune cells were identified, each containing distinct subpopulations. Cell type predictions and signature genes identified using Drop-seq were cross-validated using an independent single cell isolation platform. Temporal changes in RNA expression patterns were compared before and after birth to identify signaling pathways selectively activated in specific pulmonary cell types, demonstrating activation of UPR signaling during perinatal adaptation of the lung. Present data provide the first single cell view of the adaptation to air breathing after birth. All data from the present study are freely accessed at https://research.cchmc.org/pbge/lunggens/SCLAB.html.

2019-01-06 | GSE122329 | GEO

Preparing for the first breath at single cell level [single cell Fluidigm C1]

Project description:Preparing for the first breath at single cell level [single cell Fluidigm C1]

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Preparing for the first breath at single cell level [whole lung time course]

Project description:Preparing for the first breath at single cell level [whole lung time course]

| PRJNA504645 | ENA

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2019-01-06 | GSE122330 | GEO

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2019-01-06 | GSE122331 | GEO

Preparing for the first breath at single cell level

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-01-06 | GSE122332 | GEO

Drop-Seq analysis of P14 mouse retina single-cell suspension

Project description:A cell suspension was prepared from wild-type P14 mouse retinas, and single-cell mRNAseq libraries were generated with Drop-Seq. Drop-Seq was performed on four separate days using the same age (P14) and strain (C57BL/6). On day 1, replicate 1 was obtained. On day 2, replicates 2 and 3 were obtained. On day 3, replicates 4-6 were obtained. On day 4, replicate 7 was obtained.

2015-05-20 | E-GEOD-63472 | biostudies-arrayexpress

Drop-seq analysis of bleomycin-treated and control mouse lung single-cell suspensions

Project description:Cell suspensions were prepared from mouse lungs dissociated at baseline or 14 days after intratracheal bleomycin injury, and single cell mRNA-seq libraries were generated with Drop-seq.

2018-10-25 | E-MTAB-7142 | biostudies-arrayexpress

Drop-Seq analysis of P14 mouse retina single-cell suspension

Project description:A cell suspension was prepared from wild-type P14 mouse retinas, and single-cell mRNAseq libraries were generated with Drop-Seq.

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