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WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis

ABSTRACT: WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis

PROVIDER: PRJNA505703 | ENA |

REPOSITORIES: ENA

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WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis [RNA-seq]

Project description:During development and growth, dynamic signals need to be translated into spatially precise and temporally stable gene expression states, which define cell fate. In the context of the apical plant stem cell system, local accumulation of the small, highly mobile phytohormone auxin triggers organ initiation. Here we show that the WUSCHEL transcription factor locally protects stem cells from differentiation by controlling the auxin signaling and response pathways via regulation of Histone acetylation. Conversely, low levels of signaling are required for stem cell maintenance, demonstrating that WUSCHEL acts as a rheostat on the auxin pathway. Our results reveal an important mechanism that allows cells to differentially translate a potent and highly mobile developmental signal into appropriate cell behavior with high spatial precision and temporal robustness.

2019-11-11 | GSE122610 | GEO

WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis [ChIP-seq]

2019-11-11 | GSE122579 | GEO

WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis [ChIP-seq]

Project description:WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis [ChIP-seq]

| PRJNA505666 | ENA

WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis [RNA-seq]

Project description:WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis [RNA-seq]

| PRJNA505705 | ENA

WUSCHEL acts as an auxin response rheostat to maintain apical stem cells in Arabidopsis.

Project description:To maintain the balance between long-term stem cell self-renewal and differentiation, dynamic signals need to be translated into spatially precise and temporally stable gene expression states. In the apical plant stem cell system, local accumulation of the small, highly mobile phytohormone auxin triggers differentiation while at the same time, pluripotent stem cells are maintained throughout the entire life-cycle. We find that stem cells are resistant to auxin mediated differentiation, but require low levels of signaling for their maintenance. We demonstrate that the WUSCHEL transcription factor confers this behavior by rheostatically controlling the auxin signaling and response pathway. Finally, we show that WUSCHEL acts via regulation of histone acetylation at target loci, including those with functions in the auxin pathway. Our results reveal an important mechanism that allows cells to differentially translate a potent and highly dynamic developmental signal into stable cell behavior with high spatial precision and temporal robustness.

| S-EPMC6841675 | biostudies-literature

Response of cauline buds to apical auxin and basal cytokinin treatment

Project description:Identification of genes regulated by apical auxin and basal cytokinin treatment of the nodal stem in cauline buds of Arabidopsis thaliana

2016-12-25 | GSE59741 | GEO

WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-11-11 | GSE122611 | GEO

E-GEOD-122610

Project description:WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis [RNA-seq]

2020-04-14 | E-GEOD-122610 | ExpressionAtlas

Analysis of the effect of auxin biosynthesis inhibitor L-AOPP on the gene expression profile (shoot apical meristem)

Project description:Recently, 2-aminoxy-3-phenylpropionic acid (L-AOPP) had been demonstrated to possess an inhibitory activity against IAA biosynthesis but the molecular basis of the action was unclear. To investigate the function of L-AOPP, we conducted microarray analysis using the shoot apical meristem (SAM) part of A. thaliana in addition to whole plants after the treatment of L-AOPP. We performed microarray analysis using the shoot apical meristem (SAM) part of A. thaliana (Columbia-0) to investigate function of 2-aminoxy-3-phenylpropionic acid (L-AOPP) in relation to inhibition of auxin biosynthesis. Total RNA was extracted from SAM part of 7-day-old seedlings grown on 1/2 MS medium containing L-AOPP (50 µM),L-AOPP (50 µM) + Indole-3-acetic acid (IAA 10 nM) or Dimethyl sulfoxide (DMSO 0.1%).

2015-12-31 | E-GEOD-30092 | biostudies-arrayexpress

TMK1-mediated transcriptional auxin signaling guides differential growth in plant

Project description:Auxin plays diverse and profound roles in plants, regulating essentially all aspects of plant growth and development. Yet how auxin achieves such functional diversity and specificity is largely unknown. Here, we address how different concentrations of auxin determine developmental responses through auxin signaling pathways, which are essential for differential growth including apical hook development. We showed that a high auxin concentration at concave side of the apical hook stimulated the C-terminus cleavage and cytosolic translocation of Transmembrane Kinase 1 (TMK1), which then phosphorylates non-canonical IAA32 and IAA34. Surprisingly, and in contrast to the previous report that auxin degrades IAA proteins through the TIR1/AFB auxin receptor to regulate gene expression, here we found that non-canonical IAA32 and IAA34 proteins were stabilized by the high auxin concentration through TMK1 cleavage to negatively regulate gene expression and inhibit growth. Thus, we demonstrated that TIR1/AFB-independent transcriptional auxin signaling regulated growth inhibition in response to the high auxin concentration.

2019-02-12 | GSE111716 | GEO

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