Project description:The goal of this sequencing project was to measure transcription levels of several Cluster A actinobacteriophages during lysogeny in order to study which genes contribute to lysogeny and superinfection immunity.
Project description:The goal of this sequencing project was to measure transcription levels of several Cluster N actinobacteriophages during different stages of their lifecycle, including lysogeny, early infection (30 minutes), and late infection (2 hours 30 minutes), in order to study which genes contribute to superinfection exclusion.
Project description:Several temperate actinobacteriophages have been identified that lack integration machinery and instead have genes with predicted partitioning functions. The goal of this sequencing project was to measure transcription levels of these extrachromosomal phages during different stages of their lifecycle, including lysogeny, early infection (30 minutes), and late infection (2 hours 30 minutes), in order to study which genes contribute to extrachromosomal stability.
Project description:The goal of this sequencing project was to measure transcription levels of Sbash and Crossroads actinobacteriophages during different stages of their lifecycle, including lysogeny, early infection (30 minutes), and late infection (2 hours 30 minutes), in order to study which genes contribute to superinfection exclusion.
Project description:The goal of this sequencing project was to measure transcription levels of CarolAnn, Blueberry, Utz and Kita actinobacteriophages during different stages of their lifecycle, including lysogeny, early infection (30 minutes), and late infection (2 hours), in order to study which genes contribute to superinfection exclusion.
Project description:Different populations of the same species survive different environments through local adaptation. Temperature is one of the most important driving forces that could result in local adaptation. Here, we studied the influence of extreme low temperature on the survival of two genetically and geographically distinct populations of the free-living Caenorhabditis briggsae. We found that Caenorhabditis briggsae strains of temperate origin had a cold resistant phenotype, while those originating from a tropical climate had reduced survival after cold treatment. Using this phenotypic difference between geographically diverse populations as a model for how species adapt to their local environment, we then analyzed the transcriptional profiles of two Caenorhabditis briggsae strains of tropical and temperate origin to find genes that are involved in survival after extreme cold. In summary, the response to the extreme low temperature that clearly distinguishes the temperate and tropical Caenorhabditis briggsae strains could serve as an excellent example for studying local adaption of species that show genetic separation associated with their geographical distribution.