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Differentially expressed genes by DMSO in mouse 2-cell embryos identified by RNA sequencing

ABSTRACT: Differentially expressed genes by DMSO in mouse 2-cell embryos identified by RNA sequencing

PROVIDER: PRJNA512623 | ENA |

REPOSITORIES: ENA

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Differentially expressed genes by DMSO in mouse 2-cell embryos identified by RNA sequencing

Project description:Since discovery of dimethyl sulfoxide (DMSO), it has been applied to the multidisciplinary studies for basic and clinic research; yet its toxicity versus biocompatibility is poorly understood, leading to our inability to discover etiology behind its paradox. Here, we found that exposure of 2% DMSO to mouse zygotes caused developmental arrest at 2/4-cell embryos, and showed a decreased total acetylation levels. Besides, H3, H4, p53, H3K9, and H3K27 acetylation levels at DMSO-exposed 2-cell embryos are significantly increased. This affected the male pronucleus more than female. Further, RNA-sequence data demonstrates that DMSO alters approximately 16.26% transcripts of 2-cell embryos. Among them, maternal and minor zygotic gene activation (ZGA) transcripts are highly increased, whereas ubiquitin-proteasome system, major ZGA, embryonic gene activation, cell cycle, and ribosomal biogenesis transcripts are significantly down-regulated. These results suggest that DMSO caused developmental arrest by disrupting maternal-to-embryonic transition due to low proteasome activity and epigenetic alternations.

2019-11-08 | GSE124598 | GEO

Differentially expressed miRNAs identified in various influenza virus infection

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Identified differentially expressed lncRNAs in type 2 diabetes patients

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Identified differentially expressed lncRNAs in Type 1 Diabetes Patients

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Differentially expressed LncRNAs and mRNA identified by SEQ analysis in colorectal cancer patients

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Differentially expressed genes analysis of wild type K562 cells treated with vandetanib and control (DMSO)

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High-throughput sequencing identified differentially expressed RNAs between MALAT1 knockdown C3H10 adipocyte and control cells

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Differentially expressed miRNAs in colorectal cancer

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Differentially expressed genes following ARID1A depletion

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Zebrafish whole embryos at 36 hpf treated with DMSO or 5uM 11,12-EET

Project description:In our previous study, we found zebrafish embryos treated with 5uM 11,12-EET (epoxyeicosatrienoic acid) had increased stem cell marker, runx1, expression in the AGM. EET also induced ectopic runx1 expression in the tail. To systematically study how EET regulates gene expression, we performed microarray analysis on EET-treated embryos. Zebrafish whole embryos were synchronized at fertilization. Embryos were grown at 28 degree overnight. 25 embryos per group were treated with DMSO or 5uM 11,12-EET starting from 24 hpf (hour post fertilization) until 36 hpf at 28 degree. The triplicates were from three different clutches of embryos, and split into DMSO v.s. EET for each clutch.

2012-07-28 | GSE39707 | GEO

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