Tyrophagus putrescentiae
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA513417 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Tyrophagus putrescentiae isolate:SZU Genome sequencing and assembly
| PRJNA706095 | ENA
Project description:Wolbachia endosymbiont of Tyrophagus putrescentiae, transcriptome assembly
| PRJNA606073 | ENA
Project description:Tyrophagus putrescentiae and associated fungi and bacteria
| PRJNA598686 | ENA
Project description:Tyrophagus fanetzhangorum Genome sequencing and assembly
| PRJNA575287 | ENA
Project description:Three populations of T. putrescentiae with a known microbiome were analyzed using label-free nanoLC-MS/MS analysis. Two homogenization procedures were performed - one with a glass homogenizer (for the 3X6 dataset) and the second with beads/shards (for the 3X3 dataset). The latter was used due to its potential higher yield of bacterial proteins.
Two raw datasets (3X6 and 3X3) from the nanoLC-MS/MS analysis are included.
Compressed (zipped) combined_txt folders of database searches in MaxQuant are included. In addition, used databases (*.fasta) are provided.
2021-08-24 | MSV000088016 | MassIVE
Project description:BackgroundTyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T. putrescentiae and one mixed population of T. putrescentiae and T. fanetzhangorum collected from different habitats.MaterialThe bacterial communities of the six mite populations from different habitats and diets were compared by Sanger sequencing of cloned 16S rRNA obtained from amplification with universal eubacterial primers and using bacterial taxon-specific primers on the samples of adults/juveniles or eggs. Microscopic techniques were used to localize bacteria in food boli and mite bodies. The morphological determination of the mite populations was confirmed by analyses of CO1 and ITS fragment genes.ResultsThe following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations). From 35 identified OTUs97, only Solitalea was identified in all populations. The next most frequent and abundant sequences were Bacillus, Moraxella, Staphylococcus, Kocuria, and Microbacterium. We suggest that some bacterial species may occasionally be ingested with food. The bacteriocytes were observed in some individuals in all mite populations. Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.ConclusionThe presence of Blattabacterium-like, Cardinium, Wolbachia, and Solitalea-like in the eggs of T. putrescentiae indicates mother to offspring (vertical) transmission. RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.
| S-EPMC4940368 | biostudies-literature