Project description:In this study we subjected a library of synthetic sRNAs to multiple rounds of ethanol selection and analyzed changes in sRNA variant abundance by high-throughput sequencing.
Project description:P300 and P720 C57BL6/J male mice were exposed to ethanol vapor (95% w/v) for 3 hours each day for a total of 5 days. Blood alcohol levels in the ethanol treated animals was 3.0mg/ml. Controls at P300 and P720 were included. Keywords: SAGE-dose response All C57BL/6 male mice were sacrificed and the cerebellum was quickly removed. Total RNA was isolated using Trizol. For the creation of the libraries, 40micrograms of total RNA was used. cDNA was synthesized on oligo dt beads. Enzyme: NlaIII Vector: pZERO-I
Project description:P300 and P720 C57BL6/J male mice were exposed to ethanol vapor (95% w/v) for 3 hours each day for a total of 5 days. Blood alcohol levels in the ethanol treated animals was 3.0mg/ml. Controls at P300 and P720 were included. Keywords: SAGE-dose response
Project description:In order to increase our understanding on the epigenetic regulation in response to abiotic stresses in plants, sRNA regulation in sugarcane plants submitted to drought stress was analyzed. Deep sequencing analysis was carried out to identify the sRNA regulated in leaves and roots of sugarcane cultivars with different drought sensitivities. An enrichment of 22-nt sRNA species was observed in leaf libraries. The pool of sRNA selected allowed the analysis of different sRNA classes (miRNA and siRNA). Twenty eight and 36 families of conserved miRNA were identified in leaf and root libraries, respectively. Dynamic regulation of miRNA was observed and the expression profile of eight miRNA was verified in leaf samples by stem-loop qRT-PCR assay. Altered miRNA regulation was correlated with changes in mRNA levels of specific targets. 22-nt miRNA triggered siRNA-candidates production by cleavage of their targets in response to drought stress. Some genes of sRNA biogenesis were down-regulated in tolerant genotypes and up-regulated in sensitive in response to drought stress. Our analysis contributes to increase the knowledge on the roles of sRNA in epigenetic-regulatory pathways in sugarcane submitted to drought stress.
Project description:Purpose: To study small RNAs expressed in A. thaliana flowers Methods: sRNA libraries were constructed as previously described (C Lu et al. 2007) and sequenced on an Illumina GAIIx or Illumina HiSeq 2000 instrument at the Delaware Biotechnology Institute. These libraries were prepared between 2010 and 2012.
Project description:Collection of various small RNA libraries of C. Reinhardtii sequenced using Illumina sRNA sequencing. Libraries are derived from various C. Reinhardtii genotypes (CC1883, CC4350, CC125, J), ecotypes, life cycles (Vegatative, Zygote) treatments and mutants. Preparation was done over a period of several years as a continuous effort to build up a comprehensive collection.