Project description:We report the first secondary metabolite, 8,8'-bijuglone, obtained from pure cultures of the slow growing Douglas fir- (Pseudotsuga menziesii var. menziesii) foliage-associated fungus Zasmidium pseudotsugae. The quinone was characterized using extensive LC/MS and NMR-based spectroscopic methods. 8,8'-Bijuglone exhibited moderate antibiotic activity against Gram-positive pathogens and weak cytotoxic activity in the NCI-60 cell line panel and in our in-house human colon carcinoma (HCT-116) cell line. An analysis of the fungal genome sequence to assess its metabolic potential was implemented using the bioinformatic tool antiSMASH. In total, 36 putative biosynthetic gene clusters were found with a majority encoding for polyketides (17), followed by non-ribosomal peptides (14), terpenes (2), ribosomal peptides (1), and compounds with mixed biosynthetic origin (2). This study demonstrates that foliage associated fungi of conifers produce antimicrobial metabolites and suggests this guild of fungi may present a rich source of novel molecules.

| S-EPMC7287617 | biostudies-literature

Genome sequencing of the endosymbiotic consortium and mitochondrion of Cinara cf. splendens/pseudotsugae species voucher 3390

Project description:Genome sequencing of the endosymbiotic consortium and mitochondrion of Cinara cf. splendens/pseudotsugae species voucher 3390

| PRJEB31197 | ENA

Amylocorticium subincarnatum strain:OMC1654 | isolate:FBCC 2451

Project description:Amylocorticium subincarnatum OMC1654 Transcriptome - Ottogen6_T

| PRJNA519430 | ENA

Cerinomyces crustulinus strain:OMC1686 | isolate:FBCC 2445

Project description:Cerinomyces crustulinus OMC1686 Transcriptome - Ottogen21_T

| PRJNA571523 | ENA

Ceriporia viridans strain:OMC1683 | isolate:FBCC 2455

Project description:Ceriporia viridans OMC1683 Transcriptome - Ottogen22_T