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Self-renewal capability of hepatocytic parental progenitor cells derived from an adult rat liver is maintained for a long term in the chemically defined medium cultured on lamini111

ABSTRACT: Self-renewal capability of hepatocytic parental progenitor cells derived from an adult rat liver is maintained for a long term in the chemically defined medium cultured on lamini111

PROVIDER: PRJNA520903 | ENA |

REPOSITORIES: ENA

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Self-renewal capability of hepatocytic parental progenitor cells derived from an adult rat liver is maintained for a long term in the chemically defined medium cultured on lamini111

Project description:We analyzed gene expression of hepatocytic parental progenitor cells cultured on laminin (LN) 111, LN511 and Matrigel (Mat) in a population of small hepatocytes.

2019-02-05 | GSE126063 | GEO

Hepatocytic parental progenitor cells of rat small hepatocytes

Project description:We analyzed gene expression of hepatocytic parental progenitor cells in a population of small hepatocytes. The hepatocytic parental progenitor cells possess self-renewal capability. These cells maintain the ability to differentiate into mature hepatocytes.

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Self-renewal of hepatoblasts under chemically defined conditions by reiterative growth factor and chemical screening

Project description:Throught an reiterative growth factor and chemical screening, we defined a small molecule and growth factor cocktail, including EGF, glycogen synthase kinase 3 inhibitor (CHIR99021), transforming growth factor β receptor inhibitor (e.g. E-616452), lysophosphatidic acid and sphingosine 1-phosphate, that can sustain long-term self-renewal of murine hepatoblasts under chemically defined conditions. The expandable hepatoblasts (eHBs) by this small molecule and growth factor cocktail expressed a set of genes typical of liver progenitor cells and liver development, and retained the ability to respond to liver developmental cues and produce functional hepatocytes and form bile duct-like structures. Moreover, both early- and late-passage eHBs demonstrated a similar transcriptome profile. Microarray analysis also confirmed that the gene expression of cultured cells resembled hepatoblasts.

2014-12-29 | GSE61119 | GEO

Transcription profiling of human embryonic stem cells and their differentiated progeny grown in chemically defined conditions

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Self-Renewal Capability of Hepatocytic Parental Progenitor Cells Derived From Adult Rat Liver Is Maintained Long Term When Cultured on Laminin 111 in Serum-Free Medium.

Project description:In this study, we investigated how the ability of hepatocytic parental progenitor cells (HPPCs) to self-renew can be maintained and how laminin (LN) isoforms play an important role in their self-renewal and maturation. Hepatocytes isolated from adult rat livers were cultured on hyaluronic acid to form colonies consisting of CD44+ small hepatocytes, which could be passaged on dishes coated with Matrigel. When second-passage cells were plated on Matrigel, LN111, or LN511, HPPCs appeared on Matrigel and LN111 but not on LN511. We identified two types of cells among the second-passage cells: Small, round cells and large, flat ones were observed on Matrigel, whereas the former and latter ones were specifically attached on LN111 and LN511, respectively. We hypothesized that small and round cells are the origin of HPPC colonies, and the binding to LN111 could be key to maintaining their self-renewal capability. Among the integrins involved in LN binding, integrins ?3 and ?1 were expressed in colonies on LN111 more than in those on LN511, whereas ?4 was more strongly expressed in colonies on LN511. Integrin ?3high?6?1high cells could form HPPC colonies on LN111 but not on LN511, whereas integrin ?6?1low cells could not on either LN111 or LN511. In addition, neutralizing anti-integrin ?1 and anti-LN111 antibodies inhibited the passaged cells' ability to attach and form colonies on LN111 by HPPCs. Matrigel overlay induced second-passage cells growing on LN111 to increase their expression of hepatic functional genes and to form 3-dimensional colonies with bile canalicular networks, whereas such a shift was poorly induced when they were grown onLN511. Conclusion: These results suggest that the self-renewal capability of HPPCs depends on LN111 through integrin ?1 signaling.

| S-EPMC6939498 | biostudies-literature

Transcription profling of human embryonic stem cells differentiated into endoderm progenitors in chemically-defined media.

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Human dental pulp cells cultured in FBS vs. chemically defined conditions

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2014-12-25 | GSE59493 | GEO

Hepatocytic parental progenitor cells of rat small hepatocytes maintain self-renewal capability after long-term culture.

Project description:The liver has a variety of functions for maintaining homeostasis, and hepatocytes play a major role. In contrast with the high regenerative capacity of mature hepatocytes (MHs) in vivo, they have not been successfully expanded ex vivo. Here we demonstrate that CD44-positive cells sorted from small hepatocyte (SH) colonies derived from a healthy adult rat liver can proliferate on a Matrigel-coated dish in serum-free chemically defined medium; in addition, a subpopulation of the cells can divide more than 50 times in a period of 17 weeks every 4-week-passage. The passage cells retained the capability to recover highly differentiated functions, such as glycogen storage, CYP activity and bile secretion. When Matrigel-treated cells from the third passage were transplanted into retrorsine/partial hepatectomy-treated rat livers, the cells engrafted to differentiate into MHs and cholangiocytes. These results suggest that long-term cultured CD44+ SHs retain hepatocytic characteristics in vitro and the capability to differentiate into hepatocytes and cholangiocytes in vivo. Thus, a newly identified subpopulation of MHs possessing the attributes of hepatocytic stem/progenitor cells can be passaged several times without losing hepatocytic characteristics.

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Porcine oocytes matured in a chemically defined medium are transcriptionally active

Project description:The statement that fully-grown porcine oocytes are transcriptionally quiescent is not as strong as before. Currently, we know that there is a difference in the transcription profile of germinal vesicle (GV) and metaphase II (MII) oocytes. The goal of our study was to compare the transcription profile of GV, germinal vesicle breakdown (GVBD), metaphase I (MI), and metaphase (MII) oocytes cultivated in a chemically defined, FLI medium. Oocytes were sequenced and subsequently validated using quantitative reverse transcription polymerase chain reaction (RT-qPCR).

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Transcriptional response of Streptococcus mutans in chemically defined medium (CDM) towards the autoinducers CSP and XIP

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2015-06-01 | GSE65982 | GEO

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