Project description:To optimize the golden snub-nosed monkey genome annotation, one RNA library of mixed tissues from one individual were constructed using the Illumina mRNA-Seq Prep Kit.
Project description:Spear-ATAC is a modified droplet-based single-cell ATAC-seq (scATAC-seq) protocol that enables simultaneous read-out of chromatin accessibility profiles and integrated sgRNA spacer sequences from thousands of individual cells at once. Spear-ATAC profiling of 104,592 cells representing 414 sgRNA knock-down populations across three experiments revealed the temporal dynamics of epigenetic responses to regulatory perturbations in cancer cells and the associations between transcription factor binding profiles, demonstrating a high-throughput method for perturbing and evaluating dynamic single-cell epigenetic states.
2021-03-14 | GSE168851 | GEO
Project description:Conservation genomics of the threatened Pilbara leaf-nosed bat
Project description:Lifespan varies both within and across species, but the general principles of its control are not understood. To identify transcriptomic signatures of mammalian longevity, we sequenced multiple organs of young adult mammals corresponding to 8 different species, including Canadian beaver, long-tailed macaque, greater tube-nosed bat, baboon, white-footed mouse, sugar glider, Siberian chipmunk and American black bear. We aggregated this dataset with publicly available pan-mammalian data and performed multi-tissue gene expression analyses across 41 mammalian species. This allowed us to identifiy signatures of species longevity and assess their relationship with biomarkers of aging and lifespan-extending interventions. This dataset complements RNAseq profiles of tissues from 23 mammalian species stored at GSE43013.
