Project description:B cells belong to the adaptive immune system and have multiple functions. Besides their well characterized role in the promotion of the immune response, specific B cell subsets were shown to have an anti-inflammatory function. These cells are termed regulatory B cells and they exert their function mainly through the production of interleukin 10 (IL-10). This study focuses on B cells capable of producing IL-10 after 5 hours of stimulation with LPS, PMA and ionomycin, without any distinction based on surface markers.
Project description:Chromatin profiling of mouse splenic ex-vivo IL-10+, IL-10-CD19+CD21hiCD23hiCD24hi B cells and IL-10- follicular B cells, after antigen induced arthritis. IL-10eGFP reporter (Vert-X) mice were used to sort IL-10+ and IL-10- subsets. ATAC-seq was used in conjunction with microarray data to understand the chromatin profiles and transcriptional landscape of mouse regulatory B cells, compared to other B cell subsets.
Project description:We compared the clonotypes of IL-10+ and IL-10- B1a, marginal zone and follicular B cells from the spleen of sIgM-/-IL10GFP mouse using 10X single cell RNAseq to analyze potential differences in clonality between IL-10+ and IL-10- B cells.
Project description:IL-10 or IL-6 stimulation of control 129xC57BL/6 murine bone marrow derived macrophages in the presence of LPS. We used microarrays to detail the global programme of gene expression changes in response to IL-6 or IL-10 stimulation in the presence of lipopolysaccharide. BMDMs were isolated from control, IL-6-/-, and IL-10-/- mice on a 129XBL/6 mixed background mice and differentiated in the presence of CSF-1 for 6-7 days. Cells were scraped and plated in 6 well plates at 2x10e6/well. Cells were washed with complete DMEM and rested for 1-2 hr before stimulation with combinations of IL-10 (10 ng/ml), IL-6 (2 ng/ml) or LPS (100 ng/ml) for 45 min or 180 mins. Complete biological replicates were performed. Keywords: time course
Project description:The mechanisms in regulating the development of IL-10 producing B cells are largely unknown. To clarify which transcription factor is critical for regulation of IL-10 producing B cells, we compared the gene expression profile of IL-10 positive B cells and IL-10 negative B cells. Transcriptional repressor Prdm1/Blimp1 is known to play a key role in controlling B ells differentiation. We show a dual role for Blimp-1 in IL-10 expression in IL-10 producing B cells and plasmablasts.
Project description:During chronic schistosome infections, a complex regulatory network is induced to regulate the host immune system, in which IL-10-producing regulatory B (Breg) cells play a significant role. Schistosoma mansoni soluble egg antigens (SEA) are bound and internalized by B cells and induce both human and mouse IL-10 producing Breg cells. To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 active fractions (out of 18) in the high, medium and low MW range. The high MW fractions were rich in heavily glycosylated molecules, including multi-fucosylated proteins. Using SEA glycoproteins purified by affinity chromatography and synthetic glycans coupled to gold nanoparticles, we investigated the role of these glycan structures in inducing IL-10 production by B cells. Then, we performed proteomics analysis on active low MW fractions and identified a number of proteins with putative immunomodulatory properties, notably thioredoxin (SmTrx1) and the fatty acid binding protein Sm14. Subsequent splenic murine B cell stimulations with recombinant SmTrx1 and Sm14 showed their ability to dose-dependently induce IL-10 production and FoxP3 Treg cell priming by B cells. Identification of unique Breg cells-inducing molecules may pave the way to innovative therapeutic strategies for inflammatory and auto-immune diseases.
Project description:We cultured splenic NK cells in either low (10 ng/ml) or high (100 ng/ml) doses of IL-15 for ~72 hours. We then performed ATAC-seq to compare differentially accessible regions.
Project description:Transcriptional profiling and gene expression profiling analysis of sorted CD8+IL-10+ T cells compared to CD8+IL-10- T cells using IL-10-GFP(tiger) reporter mice