Vigna radiata
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA521836 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Comparitive transcriptome profiling of resistant and susceptible mungbean genotypes in response to infection by mungbean yellow mosaic virus
| PRJNA742191 | ENA
Project description:Identification of SIF gene via high throughput RNAseq data in mungbean
| PRJNA392002 | ENA
Project description:Genetic control of compound leaf development in mungbean (Vigna radiata L.)
| PRJNA391993 | ENA
Project description:Vigna radiata var. radiata Raw sequence reads
| PRJNA755168 | ENA
Project description:Vigna radiata cultivar:Zhonglu-1 Raw sequence reads
| PRJNA327304 | ENA
Project description:Alternative splicing (AS) can produce multiple mature mRNAs from the same primary transcript, thereby generating diverse proteins and phenotypes from the same gene. To assess the prevalence of AS in mungbean (Vigna radiata), we analysed whole-genome RNA sequencing data from root, leaf, flower and pod tissues and found that at least 37.9% of mungbean genes are subjected to AS. The number of AS transcripts exhibited a strong correlation with exon number and thus resembled a uniform probabilistic event rather than a specific regulatory function. The proportion of frameshift splicing was close to the expected frequency of random splicing. However, alternative donor and acceptor AS events tended to occur at multiples of three nucleotides (i.e. the codon length) from the main splice site. Genes with high exon number and expression level, which should have the most AS if splicing is purely stochastic, exhibited less AS, implying the existence of negative selection against excessive random AS. Functional AS is probably rare: a large proportion of AS isoforms exist at very low copy per cell on average or are expressed at much lower levels than default transcripts. Conserved AS was only detected in 629 genes (2.8% of all genes in the genome) when compared to Vigna angularis, and in 16 genes in more distant species like soya bean. These observations highlight the challenges of finding and cataloguing candidates for experimentally proven AS isoforms in a crop genome.
| S-EPMC5258860 | biostudies-other