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Worker antennal gene expression in Solenopsis invicta identifies candidate genes for queen discrimination

ABSTRACT: Worker antennal gene expression in Solenopsis invicta identifies candidate genes for queen discrimination

PROVIDER: PRJNA522987 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8570220_1.fastq.gz	Fastqsanger.gz
	SRR8570220_2.fastq.gz	Fastqsanger.gz
	SRR8570230_1.fastq.gz	Fastqsanger.gz
	SRR8570230_2.fastq.gz	Fastqsanger.gz
	SRR8570221_1.fastq.gz	Fastqsanger.gz

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Worker antennal gene expression in Solenopsis invicta identifies candidate genes for queen discrimination (Clontech_lib_RNA-seq)

Project description:Queen discrimination behavior in the red imported fire ant Solenopsis invicta maintains its two types of societies: colonies with one (monogyne) or many (polygyne) queens, yet the underlying genetic mechanism is poorly understood. This behavior is controlled by two supergene alleles, SB and Sb, with ~600 genes. Polygyne workers, having either the SB/SB or SB/Sb genotype, accept additional SB/Sb queens into their colonies but kill SB/SB queens. While monogyne workers, all SB/SB, reject all additional queens regardless of genotype. Because the SB and Sb alleles do not recombine, it is difficult to determine which genes within the supergene mediate this differential worker behavior. We hypothesized that the alternate worker genotypes sense queens differently because of different patterns of gene expression in their main sensory organ, the antennae. To identify such differentially expressed genes, we sequenced RNA from four biological replicates of pooled antennae from three groups of workers: monogyne SB/SB, polygyne SB/SB, and polygyne SB/Sb. We identified 81 differentially expressed protein coding genes with 14 encoding potential odor metabolism and perception proteins. We focused on the two differentially expressed odorant perception genes: an odorant binding protein SiOBP12 and an odorant receptor SiOR463. We found that the SiOR463 was lost in the Sb-genome. In contrast, the SiOBP12 has an Sb-specific duplication SiOBP12b’, which was expressed in the SB/Sb worker antennae, while both paralogs SiOBP12 and SiOBP12b’ were expressed in the body. This result indicates that SiOBP12b’ has gained an antennal promoter or enhancer and suggests neofunctionalization, perhaps for queen discrimination behavior.

2020-04-10 | GSE126673 | GEO

Worker antennal gene expression in Solenopsis invicta identifies candidate genes for queen discrimination (Nugen_lib_RNA-seq-2)

2020-04-10 | GSE126674 | GEO

Worker antennal gene expression in Solenopsis invicta identifies candidate genes for queen discrimination (Nugen_lib_RNA-seq-1)

2020-04-10 | GSE126368 | GEO

Worker antennal gene expression in Solenopsis invicta identifies candidate genes for queen discrimination (Clontech_lib_RNA-seq)

Project description:Worker antennal gene expression in Solenopsis invicta identifies candidate genes for queen discrimination (Clontech_lib_RNA-seq)

| PRJNA522913 | ENA

Worker antennal gene expression in Solenopsis invicta identifies candidate genes for queen discrimination (Nugen_lib_RNA-seq-1)

Project description:Worker antennal gene expression in Solenopsis invicta identifies candidate genes for queen discrimination (Nugen_lib_RNA-seq-1)

| PRJNA522069 | ENA

Worker antennal gene expression in Solenopsis invicta identifies candidate genes for queen discrimination (Nugen_lib_RNA-seq-2)

Project description:Worker antennal gene expression in Solenopsis invicta identifies candidate genes for queen discrimination (Nugen_lib_RNA-seq-2)

| PRJNA522912 | ENA

Comparison of fire ant adult queens with different supergene genotypes (SB/SB and SB/Sb) and different age classes (1-day-old virgins, 11-day-old virgins and reproductive queens) from 2008 and 2009

2012-11-07 | GSE42062 | GEO

Worker antennal gene expression in Solenopsis invicta identifies candidate genes for queen discrimination

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-04-10 | GSE126684 | GEO

Transcriptional profiling by array of Solenopsis invicta (fire ant) workers to identify genes and gene pathways responding to the queen presence

Project description:We used whole-genome fire ant microarrays to examine the molecular basis for social organization in Solenopsis invicta. Monogyne (single queen) fire ant colonies were collected in the field and transported into the lab were they were reared in standard conditions for two weeks. At this point, each colony was split into two sub-colonies: one sub-colony contained the functional mother queen (queenright) while the other was left queenless. Each sub-colony included a nesting chamber, containing the brood and workers performing nursing tasks, and a foraging area, separated from the nesting chamber and provided with food and water sources. For both queenright and queenless sub-colonies, foraging workers were collected in the foraging area while non-foraging workers were collected in the nesting chamber. Total RNA was isolated from pools of 10 whole workers and processed for microarrays.

2014-01-08 | E-MTAB-2108 | biostudies-arrayexpress

Comparison of fire ant adult queens with different supergene genotypes (SB/SB and SB/Sb) and different age classes (1-day-old virgins, 11-day-old virgins and reproductive queens) from 2008 and 2009

Project description:In the fire ant Solenopsis invicta, a colony queen number is determined by the founding queen's genotypes at the 13 Mb supergene with the non-recombining variants SB and Sb. Single-queen colonies are always headed by SB/SB queens while multiple-queens colonies are always headed by SB/Sb queens. The two variants of the supergene, SB and Sb are completely linked to the two alleles (B and b) of the gene Gp-9. SB/SB and SB/Sb queens differ in many physiological traits including their maturation rate and odor. To explain why SB/SB and SB/Sb queens have different odors, and why SB/SB virgins mature faster and accumulate more fat, we measured expression of ~6000 genes in virgin queens 1 and 11 days after eclosion and in reproductive queens. Keywords: fire ants, Solenopsis invicta, Supergene, queen, Gp-9, social form, maturation, fat storage, queen odor, cuticular hydrocarbon, worker discrimination, monogyne, polygyne, transposon, chemical signaling Six-condition experiment: 1-day-old SB/SB virgins, 1-day-old SB/Sb virgins, 11-day-old SB/SB virgins, 11-day-old SB/Sb virgins, SB/SB reproductive queens, SB/Sb reproductive queens. Biological replicates: 8 for 1-day-old SB/SB virgins and 1-day-old SB/Sb virgins that were collected in 2008; 8 for 1-day-old SB/SB virgins, 1-day-old SB/Sb virgins that were collected in 2009; 7 for 11-day-old SB/SB virgins and 11-day-old SB/Sb virgins that were collected in 2008; 8 for 11-day-old SB/SB virgins and 11-day-old SB/Sb virgins that were collected in 2009; 8 for SB/SB and SB/Sb reproductive queens (only collected in 2009). Samples were labeled with Cy3 and were compared to the same common reference RNA labeled with Cy5. Samples from 2008 were hybridized on the microarrays batch I and samples from 2009 were hybridized on the microarrays batch J.

2012-11-07 | E-GEOD-42062 | biostudies-arrayexpress

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