Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Manis pentadactyla isolate:MP20

ABSTRACT: Manis pentadactyla isolate:MP20 Genome sequencing and assembly

PROVIDER: PRJNA529513 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR8992852_1.fastq.gz	Fastqsanger.gz
	SRR8992852_2.fastq.gz	Fastqsanger.gz
	SRR8992843_1.fastq.gz	Fastqsanger.gz
	SRR8992843_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 27

Similar Datasets

Manis pentadactyla

Project description:Manis pentadactyla Genome sequencing and assembly

| PRJNA822507 | ENA

Manis javanica isolate:MJ74

Project description:Manis javanica isolate:MJ74 Genome sequencing and assembly

| PRJNA529512 | ENA

Manis javanica isolate:MP_PG03-UM

Project description:Manis javanica isolate:MP_PG03-UM Genome sequencing and assembly

| PRJNA256023 | ENA

Manis pentadactyla

Project description:Pangoling genome sequencing

| PRJNA298142 | ENA

Manis javanica

Project description:Manis javanica Genome sequencing and assembly

| PRJNA813723 | ENA

Manis crassicaudata

Project description:Manis crassicaudata Genome sequencing and assembly

| PRJNA490788 | ENA

Comparative optical genome analysis of two pangolin species: Manis pentadactyla and Manis javanica.

Project description:Background:The pangolin is a Pholidota mammal with large keratin scales protecting its skin. Two pangolin species ( Manis pentadactyla and Manis javanica ) have been recorded as critically endangered on the International Union for Conservation of Nature Red List of Threatened Species. Optical mapping constructs high-resolution restriction maps from single DNA molecules for genome analysis at the megabase scale and to assist genome assembly. Here, we constructed restriction maps of M. pentadactyla and M. javanica using optical mapping to assist with genome assembly and analysis of these species. Findings:Genomic DNA was nicked with Nt.BspQI and then labeled using fluorescently labeled bases that were detected by the Irys optical mapping system. In total, 3,313,734 DNA molecules (517.847 Gb) for M. pentadactyla and 3,439,885 DNA molecules (504.743 Gb) for M. javanica were obtained, which corresponded to approximately 178X and 177X genome coverage, respectively. Qualified molecules (?150 kb with a label density of >6 sites per 100 kb) were analyzed using the de novo assembly program embedded in the IrysView pipeline. We obtained two maps that were 2.91 Gb and 2.85 Gb in size with N50s of 1.88?Mb and 1.97?Mb, respectively. Conclusions:Optical mapping reveals large-scale structural information that is especially important for non-model genomes that lack a good reference. The approach has the potential to guide de novo assembly of genomes sequenced using next-generation sequencing. Our data provide a resource for Manidae genome analysis and references for de novo assembly. This note also provides new insights into Manidae evolutionary analysis at the genome structure level.

| S-EPMC6082621 | biostudies-literature

Manis pentadactyla

Project description:Chinese pangolin, sometimes referred to as the spiny anteater

| PRJNA20329 | ENA

Manis pentadactyla

Project description:Chinese pangolin, sometimes referred to as the spiny anteater

| PRJNA20331 | ENA

Manis javanica

Project description:Manis javanica overview

| PRJNA280344 | ENA