Project description:ATF1 encodes a sequence-specific activating TF containing a bZIP DNA binding domain, which is overexpressed in CRC tumor tissues compared with their paired normal tissues in two independent cohorts and that ATF1 amplification also frequently occurs across multiple cancer types. Moreover, ATF1 is more highly expressed in advanced stages of CRC. Mechanistically, ATF1 overexpression could provoke cell proliferation and xenograft growth in vitro and in vivo by affecting cell apoptosis, However, the exact mechanism and downstream transcriptional programs by which ATF1 provokes tumor activity are not well understood. We performed ChIP-seq in HCT116cells to investigate the regulatory mechamisms of ATF1.
Project description:Transcriptional profiling of hPTTG1-/- HCT116 human colorectal cancer cells comparing hPTTG1-/- HCT116 cells transfected with pcDNA3.1, and with hPTTG1-/- HCT116 cells transfected with pcDNA3.1-hPTTG1 plasmid.
Project description:Transcriptional profiling of hPTTG1-/- HCT116 human colorectal cancer cells comparing hPTTG1-/- HCT116 cells transfected with pcDNA3.1, and with hPTTG1-/- HCT116 cells transfected with pcDNA3.1-hPTTG1 plasmid. Two-condition experiment, pchyg vs. 7-2 and 18-2 cells. 1 control, 2 different transfected cells.
Project description:Determination of the ion channel and transporters gene expression profile of Bevacizumab-adapted colorectal adenocarcinoma cells HCT116
Project description:Transcriptional profiling of HCT116 cells transfected with either control siRNA or TET1 siRNA was analyzed using whole human genome microarrays. To identify genes regulated by TET1 in colorectal cancer, HCT116 cells were transfected with either control siRNA or TET1 siRNA, and total RNA was extracted from biologically duplicated samples.
Project description:The experiment was to study the gene expression changes in human colorectal cell HCT116 DICER Exon5 knockout cells comparing to that in parental HCT116 cellls. Experiment Overall Design: Experiment includes using of two Agilent human 44K microarrays with dye-swap replication.
Project description:The experiment was to study the gene expression changes in human colorectal cell HCT116 DICER Exon5 knockout cells comparing to that in parental HCT116 cellls. Keywords: Cell type comparison
Project description:Identification of genes and pathways that were influenced by nu7441, the PRKDC inhibitor, in HCT116 colorectal carcinoma cell line.