Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of LncRNAs and mRNAs involved in renal ishchemia reperfusion injury

ABSTRACT: Identification of LncRNAs and mRNAs involved in renal ishchemia reperfusion injury

PROVIDER: PRJNA543756 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Identification of LncRNAs and mRNAs involved in renal ishchemia reperfusion injury

Project description:The identification of the expression patterns of long non‑coding RNAs (lncRNAs) and mRNAs in the kidney under normal and renal ischemia/reperfusion (IR) conditions is essential for understanding the genetic mechanisms underlying the pathogenesis of renal IR injury. Here, we have carried out a genome-wide long non-coding RNA and mRNA microarray analysis in mice kidneys with IR. The data revealed that the expression profiles of lncRNAs and mRNAs in the kidneys differed markedly between the Sham group and IR group, and in total 276 differentially expressed (>2‑fold) lncRNAs (201 upregulated, 75 downregulated) and 267 mRNAs (192 upregulated, 75 downregulated) were identified. qRT-PCR analysis was performed to verify the expression patterns of several lncRNAs and mRNAs.We found a specific and strongly differentially expressed lncRNA, an renal ischemia-reperfusion (IR) injury associated RNA, termed lncRNA-IRAR, which was mainly located in tubular epithelia cells. In vitro and in vivo gain- or loss-of-function studies were performed to investigate the role of lncRNA-IRAR during renal IRI. Moreover, an expression volcano map of the differentially expressed mRNAs showed that UCP1 was the most dramatically downregulated gene, and its role in oxidative stress and apoptosis was assessed both in vitro and in vivo. Genetic deletion of UCP1 was used to investigate the effects of UCP1 on ischemia -indued acute kidney injury in mice.

2019-05-21 | GSE131454 | GEO

Effect of Celastrol on LncRNAs and mRNAs Profiles of Cerebral Ischemia-Reperfusion Injury in Transient Middle Cerebral Artery Occlusion Mice Model

Project description:Celastrol plays a significant role in cerebral ischemia-reperfusion injury. Although previous studies have confirmed that celastrol post-treatment has a protective effect on ischemic stroke, the therapeutic effect of celastrol on ischemic stroke and the underlying molecular mechanism remain unclear. In the present study, focal transient cerebral ischemia was induced by transient middle cerebral artery occlusion (tMCAO) in mice and celastrol was administered immediately after reperfusion. We performed lncRNA and mRNA analysis in the ischemic hemisphere of adult mice with celastrol post-treatment through RNA-Sequencing (RNA-Seq). A total of 50 differentially expressed lncRNAs (DE lncRNAs) and 696 differentially expressed mRNAs (DE mRNAs) were identified between the sham and tMCAO group, and a total of 544 DE lncRNAs and 324 DE mRNAs were identified between the tMCAO and tMCAO+celastrol group. Bioinformatic analysis was done on the identified deregulated genes through gene ontology (GO) analysis, KEGG pathway analysis and network analysis. Pathway analysis indicated that inflammation-related signaling pathways played vital roles in the treatment of ischemic stroke by celastrol. Our study suggests celastrol treatment can effectively reduce cerebral ischemia-reperfusion injury. The bioinformatics analysis of lnRNAs and mRNAs profiles in the ischemic hemisphere of adult mice provides a new perspective in the neuroprotective effects of celastrol, particularly with regards to ischemic stroke.

2022-05-13 | GSE202659 | GEO

High-throughput Sequencing contributes to Quantitative Analysis of Renal Ischemic-Reperfusion-Injury Transcriptomes

Project description:Purpose: The different ischemic time of Ischemia-reperfusion-injury (IRI) mouse models resulted in different outcomes, severe IRI led to chronic kidney disease, while mild IRI led to the recovery of kidneys. We isolated tubules from mice from each group. The goals of this study are to compare differentially expressed mRNAs, lncRNAs and circRNAs among severe IRI-injured tubules, mild IRI-injuried tubules and control mouse tubules.

2022-04-20 | GSE179506 | GEO

Alterations in the expression of long noncoding RNAs and mRNAs in extracellular vesicles derived from the murine heart post ischemia-reperfusion injury

Project description:Extracellular vesicles (EVs) play important roles in cardiovascular diseases by delivering multiple RNA molecular, such as lncRNAs and mRNA. However, the features and functional mechanisms of the lncRNAs and mRNA in cardiac EVs during ischemia-reperfusion (IR) remains poorly understood. Therefore, we performed RNA sequencing to profile the expression feature of lncRNAs and mRNAs and predict their potential functions.

2023-01-11 | GSE189888 | GEO

Autologous adipose-derived cells transiently moderate inflammation and fibrogenesis in early renal ischemia reperfusion injury

Project description:Adipose-derived regenerative cell (ADRC) heterogeneity and functionality in renal ischemia reperfusion injury

2019-10-25 | GSE139318 | GEO

Identification of LncRNAs and mRNAs involved in adipogenesis

Project description:Identification of LncRNAs and mRNAs involved in adipogenesis

| PRJNA788280 | ENA

Epigenetic changes of pericytes after ischemia-reperfusion renal injury

Project description:Analysis of epigenetic changes of pericytes after ischemia-reperfusion renal injury. The hypothesis tested in the present study was that epigenetic change develope in pericytes after acute kidney injury. This phenotype change would cause pericyte to be more proliferative and profibrotic. Results provide important information of the epigenetic change of pericytes, such as specific mechano-responsive genes, up-regulated specific proliferative and profibrotic functions.

2019-12-05 | GSE140988 | GEO

The analysis of differential gene expression after remote ischemic preconditioning of renal ischemia reperfusion injury

Project description:To investigate the mechanism by which ischemic preconditioning (IPC) produces tissue tolerance to renal ischemia reperfusion injury in a pig model

2016-09-02 | GSE72646 | GEO

ACSL4 effect on renal ischemia-reperfusion (I/R) injury

Project description:ACSL4 effect on renal ischemia-reperfusion (I/R) injury

| PRJNA753588 | ENA

Epigenetic changes of pericytes after ischemia-reperfusion renal injury

Project description:Epigenetic changes of pericytes after ischemia-reperfusion renal injury

| PRJNA591616 | ENA