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Phospho-antibody microarray analyses for islets of control and Mettl14 knock-out mice

ABSTRACT: Phospho-antibody microarray analyses for islets of control and Mettl14 knock-out mice

PROVIDER: PRJNA545963 | ENA |

REPOSITORIES: ENA

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Phospho-antibody microarray analyses for islets of control and Mettl14 knock-out mice

Project description:β-cell specific Mettl14 knock-out mice display reduced N6-methyladenosine (m6A) levels and recapitulate human Type II diabetes (T2D) islet phenotype with early diabetes onset and mortality secondary to decreased β-cell proliferation and insulin degranulation. To gain insights into the role of m6A in regulating the IGF1/insulin -> AKT - > PDX1 pathway and to dissect the signaling networks modulating AKT phosphorylation, we subjected freshly isolated islets from control and Mettl14 knock-out mice to phospho-antibody microarrays.

2019-08-09 | GSE132111 | GEO

Phospho-antibody microarray analyses for islets of control and IFT88 knockout mice [PEX100]

Project description:β-cell specific IFT88 knock-out mice recapitulate human diabetes with impaired insulin secretion and altered islet hormone paracrine regulation. To examine the signaling pathways regulating islet cell function, we subjected protein lysates of whole islets from control and IFT88 knockout mice to a commercial phospho-antibody array analysis (Full Moon Bio, Inc). Samples were probed against 1318 site-specific and phospho-specific antibodies with 2 replicates per antibody on 76 x 25 x 1mm glass slides.

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Phospho-antibody microarray analyses for islets of control and IFT88 knockout mice [PEX100]

Project description:Phospho-antibody microarray analyses for islets of control and IFT88 knockout mice [PEX100]

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m6A-sequencing of Mettl14-conditional knock-out retinas and matched control.

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Phospho-antibody microarray anayses for control and DG-LRG1 treated HUVEC cells.

Project description:Deglycosylated-leucine-rich α-2-glycoprotein1 (DG-LRG1) as well as LRG1 was discovered to promote angiogenesis under diabetes mellitus condition through TGF-β independent binding to endoglin. To examine the signaling pathways triggered by DG-LRG1, we subjected whole-cell protein lysates of control and DG-LRG1 treated HUVECs to a Phospho Explorer antibody array analysis using commercial antibody array assay kit (Full Moon Biosystems, Inc.). Samples were probed against 1318 site-specific and phospho-specific antibodies with two replicates per antibody printed on a coated glass microscope slide. Phospho Explorer antibody array experiments and analyses were performed as a custom service by E-Biogen (Ebiogen Inc., Seoul, Republic of Korea).

2020-10-25 | GSE160005 | GEO

Single-cell RNA-sequencing of Mettl14 conditional knock-out retinas and matched controls

Project description:Post-transcriptional m6A methylation on mRNA plays a key role in neural development. Here, we specifically depleted Mettl14 (a key component of m6A complex) in retina progenitor cells by crossing Mettl14 fl/fl mice with the Chx10-Egfp/Cre mouse line and investigated cell type and transcriptome changes in the developing retinas using scRNA-seq.

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Phospho-antibody microarray analyses for wild-type and Pak1-deficient breast cancer cells

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Identifying metabolite changes in human islets treated with Phospho-BAD mimicry and Inflammatory cytokines

Project description:The goal of this study was to associate metabolite changes with protection of human islets from cell death induced by the diabetogenic stress of inflammatory cytokines. Protection of human islet viability was accomplished via enhanced glucose metabolism using phospho-BAD mimicry peptide treatment.

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Expression profiling of pancreatic islets in Tcf1 knock out mice

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PI3K/mTOR specific phospho-antibody microarray to study the effect of rapamycin to the innate immune respsonse

Project description:To comprehensively analyze the effects of mTORC1 inhibition on GSK3, we employed the use of a PI3K/mTOR-specific phospho-antibody microarray that analyzed the site-specific phosphorylation of over 130 kinases within the PI3K/mTOR pathway. The phosphorylation levels of different kinases in monocytes were measured when stimulated with LPS in the presence or absence of a kind of mTORC1 inhibitor, rapamycin More than 130 highly specific and characterized phospho-antibodies for the human mTOR signaling pathway were immobilized and replicated six times on glass slides. The same non-phosphorylated target antibodies were included to allow the determination of the relative level of phosphorylatioin

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