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Phospho-antibody microarray analyses for islets of control and Mettl14 knock-out mice

ABSTRACT: Phospho-antibody microarray analyses for islets of control and Mettl14 knock-out mice

PROVIDER: PRJNA545963 | ENA |

REPOSITORIES: ENA

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Phospho-antibody microarray analyses for islets of control and Mettl14 knock-out mice

Project description:β-cell specific Mettl14 knock-out mice display reduced N6-methyladenosine (m6A) levels and recapitulate human Type II diabetes (T2D) islet phenotype with early diabetes onset and mortality secondary to decreased β-cell proliferation and insulin degranulation. To gain insights into the role of m6A in regulating the IGF1/insulin -> AKT - > PDX1 pathway and to dissect the signaling networks modulating AKT phosphorylation, we subjected freshly isolated islets from control and Mettl14 knock-out mice to phospho-antibody microarrays.

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Phospho-antibody microarray analyses for islets of control and IFT88 knockout mice [PEX100]

Project description:β-cell specific IFT88 knock-out mice recapitulate human diabetes with impaired insulin secretion and altered islet hormone paracrine regulation. To examine the signaling pathways regulating islet cell function, we subjected protein lysates of whole islets from control and IFT88 knockout mice to a commercial phospho-antibody array analysis (Full Moon Bio, Inc). Samples were probed against 1318 site-specific and phospho-specific antibodies with 2 replicates per antibody on 76 x 25 x 1mm glass slides.

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Phospho-antibody microarray analyses for islets of control and IFT88 knockout mice [PEX100]

Project description:Phospho-antibody microarray analyses for islets of control and IFT88 knockout mice [PEX100]

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m6A-sequencing of Mettl14-conditional knock-out retinas and matched control.

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Phospho-antibody microarray anayses for control and DG-LRG1 treated HUVEC cells.

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2020-10-25 | GSE160005 | GEO

Single-cell RNA-sequencing of Mettl14 conditional knock-out retinas and matched controls

Project description:Post-transcriptional m6A methylation on mRNA plays a key role in neural development. Here, we specifically depleted Mettl14 (a key component of m6A complex) in retina progenitor cells by crossing Mettl14 fl/fl mice with the Chx10-Egfp/Cre mouse line and investigated cell type and transcriptome changes in the developing retinas using scRNA-seq.

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Phospho-antibody microarray analyses for wild-type and Pak1-deficient breast cancer cells

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Identifying metabolite changes in human islets treated with Phospho-BAD mimicry and Inflammatory cytokines

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PI3K/mTOR specific phospho-antibody microarray to study the effect of rapamycin to the innate immune respsonse

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