Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell qRT-PCR (scPCR) for rat hepatocytes and biliary epithelial cell (BEC)-enriched non-parenchymal cells (NPCs).

ABSTRACT: Single cell qRT-PCR (scPCR) for rat hepatocytes and biliary epithelial cell (BEC)-enriched non-parenchymal cells (NPCs).

PROVIDER: PRJNA548128 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Single cell qRT-PCR (scPCR) for rat hepatocytes and biliary epithelial cell (BEC)-enriched non-parenchymal cells (NPCs).

Project description:Using FACS-sorted adult rat hepatocytes with distinct DNA contents (2c and 4c), we compared the expression levels of a 47 gene panel. As non-hepatic control samples, BEC-enriched NPCs were also used for the same scPCR experiment.

2019-08-01 | GSE132459 | GEO

Phenotype and function of ex vivo expanded liver progenitor-like cells through biliary epithelial cells reversion

Project description:The current study combined genetic lineage tracing with in vitro small-molecule-based reprogramming to define liver progenitor cells (LPCs) derived from hepatic parenchymal and non-parenchymal cells (NPCs). tdTom+ hepatocytes were isolated from ROSA26tdTomato mice following AAV8-Tbg-Cre-mediated recombination, ALB/GFP-EpCAM- NPCs were isolated from AlbCreERT/R26GFP mice and EpCAM+ biliary epithelial cells (BECs) from wild-type intrahepatic bile ducts. A cocktail of small molecules was used to convert the isolated cells into LPCs.

2024-07-24 | GSE238160 | GEO

A Single-Cell Atlas of Murine Liver Non-parenchymal Cells in Typical Disease Models

Project description:We provided a comprehensive single-cell transcriptomic atlas of murine liver non-parenchymal cells (NPCs) in different diseases and analyzed the heterogeneity of inter- and intra-group NPCs. Our study offers a valuable resource for better understanding liver physiology and pathology, and bring new directions for the development of clinical diagnostic markers and effective therapeutic targets.

2021-08-01 | GSE166178 | GEO

mRNA expression profiling of ERCP biliary brushings of malignant biliary strictures

Project description:Identification of differential gene expression uging endoscopic biliary brushings Bile duct brishings of benign biliary strictures and malignant biliary strictures, samples snap frozen in liquid nitrogen

2012-03-01 | E-GEOD-34166 | biostudies-arrayexpress

Mock and stably infected rKSHV.219 cells (LEC and BEC) [Expt1]

Project description:Expression profiling of stably infected primary endothelial cells using a custom tiling microarray. LEC and BEC were infected with rKSHV.219 and selected with puromycin for 2 weeks. Mock infected LEC and BEC served as controls for their infected counterparts. All cells were harvested after 2 weeks of stable infection.

2013-03-29 | GSE45590 | GEO

Single-cell RNA sequencing to reveal non-parenchymal cell heterogeneity and immune network of acetaminophen-induced liver injury in mice

Project description:The role of non-parenchymal cells (NPCs) in the early phase of acetaminophen (APAP)-induced liver injury (AILI) remains unclear. Therefore, single-cell sequencing (scRNA-seq) was performed to explore the heterogeneity and immune network of NPCs in the livers of mice with AILI. Mice were challenged with saline, 300 mg/kg APAP, or 750 mg/kg APAP (n=3 for each group). After 3 h, the liver samples were collected, digested, and subjected to scRNA-seq. Immunohistochemistry and immunofluorescence were performed to confirm the expression of Makorin ring finger protein 1 (Mkrn1). We identified 14 distinct cell subtypes among the 120,599 cells. A variety of NPCs were involved even in the early stages of AILI, indicating highly heterogeneous transcriptome dynamics. Cholangiocyte cluster 3 with high Deleted in malignant brain tumors 1 (Dmbt1) expression was found to perform drug metabolism and detoxification functions. Liver sinusoidal endothelial cells displayed fenestrae loss and angiogenesis. Macrophage cluster 1 displayed an M1 polarization phenotype, whereas cluster 3 tended to exhibit M2 polarization. Kupffer cells (KCs) particularly highly expressed Cxcl2 exhibiting pro-inflammatory effects. qRT-PCR and western blotting verified that LIFR-OSM axis might promote the activation of MAPK signaling pathway in RAW264.7 macrophages. Mkrn1 was highly expressed in the liver macrophages of AILI mice and patients with AILI. Interaction patterns between macrophages/KCs and other NPCs were complex and diverse. NPCs were highly heterogeneous and involved in the immune network in the early phase of AILI. In addition, we proposed that Mkrn1 may serve as a potential biomarker of AILI.

2023-09-30 | GSE228305 | GEO

Mock and stably infected rKSHV.219 cells (LEC, BEC, HUVEC, and HAEC) [Expt2]

Project description:Expression profiling of stably infected primary endothelial cells using a custom tiling microarray. LEC, BEC, HUVEC, and HAEC were infected with rKSHV.219 and selected with puromycin for 2 weeks. Mock infected LEC, BEC, HUVEC, and HAEC served as controls for their infected counterparts. All cells were harvested after 2 weeks of stable infection.

2013-03-29 | GSE45591 | GEO

VEGFA mRNA-LNP promote biliary epithelial cell-to-hepatocyte conversion in acute and chronic liver diseases and reverses steatosis and fibrosis

Project description:The goal of this experiment was to analyse transcriptomic changes between Biliary epithelial cells or BECs from the liver of mice treated with Acetaminophen (a drug that causes acute liver injury) in combination with AAV8-Tbg-p21 viral vector to induce hepatocyte senecence. The aim was to understand the impact of injury on transcriptional status of biliary epithelial cells. Briefly, we analyzed mouse liver cells (hepatocytes and non-parenchymal cells fractions) obtained by in-situ liver perfusion 48 h after injury. We further extracted cells corresponding to the hepatocyte and biliary epithelial cell gene signature for detailed analysis. Two separate clusters of BECs (BEC1 and BEC2) were further identified and analyzed to study their transcriptional changes and corresponding pathways.

2023-11-17 | GSE242847 | GEO

Burkholderia pseudomallei strain:BEC

Project description:Burkholderia pseudomallei strain:BEC Genome sequencing and assembly

| PRJNA244253 | ENA

MicroRNA expression in human lymphatic endothelial cells (LEC) and blood endothelial cells (BEC)

Project description:This experiment was aim to identify differentially expressed miRNAs from cultured human LEC and BEC using microRNA-seq.

2019-02-19 | GSE126679 | GEO