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Genome-wide bisulfite sequencing of embryos at E4.5 stage injected with sRNAs from normal and stress-induced depressed mice

ABSTRACT: Genome-wide bisulfite sequencing of embryos at E4.5 stage injected with sRNAs from normal and stress-induced depressed mice

PROVIDER: PRJNA551621 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9610942_1.fastq.gz	Fastqsanger.gz
	SRR9610942_2.fastq.gz	Fastqsanger.gz
	SRR9610943_1.fastq.gz	Fastqsanger.gz
	SRR9610943_2.fastq.gz	Fastqsanger.gz
	SRR9610944_1.fastq.gz	Fastqsanger.gz

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Genome-wide bisulfite sequencing of embryos at E4.5 stage injected with sRNAs from normal and stress-induced depressed mice

Project description:We demonstrated that DNMT3a was decreased at E3.5 to E6.5 stage in embryos developing from zygotes injected with sperm sRNAs from depressed mice.To uncover the consequence caused by DNMT3a transient reduction in developing embryos, we injected sperm sRNAs from normal or depressed mice into zygotes and systematically profiled the DNA methylome of embryos at E4.5 stage (sRNA-Dep-E4.5 vs sRNA-Ctl-E4.5) by single-cell level whole genome bisulphite sequencing (SC-WGBS).

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Project description:Purpose: Searching for sRNAs in Salmonella pullorum by RNA sequencing and exploring their functions.Methods: High-throughput sequencing of RNA extracted from Salmonella pullorum under normal growth conditions to detect newly discovered sRNAs, followed by experiments to verify their functions.Results: The proportion of Clean Reads of this sequencing was >65%, and the base Q30s were all above 85%, indicating that the sequencing quality is good and can be used for subsequent analysis. The sRNAscanner software predicted that 148 new sRNAs might exist on the reference genome of Salmonella fowl dysentery, and the reads obtained from sequencing were compared to the genome, and it was found that 110 out of the 148 newly predicted sRNAs could be detected.Conclusions: sRNAs are widely found in bacteria and are involved in many physiological processes. In this study, we detected new sRNAs in Salmonella pullorum by RNA-seq, which lays the foundation for the subsequent investigation of the regulatory functions of sRNAs in bacteria.

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