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Illumina sequencing of PCR products derived from TBLV-induced tumors in the presence and absence of Rem

ABSTRACT: Illumina sequencing of PCR products derived from TBLV-induced tumors in the presence and absence of Rem

PROVIDER: PRJNA554252 | ENA |

REPOSITORIES: ENA

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Illumina sequencing of PCR products derived from TBLV-induced tumors in the presence and absence of Rem

Project description:Sequencing of portions (polymerase, envelope, and part of the 3’ LTR) of the TBLV retroviral genome were recovered by PCR amplification from tumors induced with or without Rem expression. PCR products from the mouse Gapdh gene were obtained as a control for acquisition of mutations during PCR and library preparation.

2019-07-13 | GSE134189 | GEO

The expression profiles of extracellular matrix-related genes in the presence or absence of IL-12, IL-23 or IL-27 as measured with PCR array

Project description:We performed a PCR array of 84 ECM-related genes using RNA obtained from dermal fibroblasts stimulated presence or absence of IL-12, IL-23 or IL-27. The effects of IL-12, -23 or -27 on COL1A2 mRNA expression were less than 2-fold, as compared with untreated cells.

2016-03-03 | GSE78838 | GEO

CD4+ T cells in presence or absence of Ikaros

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Project description:We report the results of high throughput sequencing on P. gingivalis cells in the presence or absence of 4 aminobenzoate/para-amino benzoic acid (pABA)

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The expression profiles of extracellular matrix-related genes in the presence or absence of IL-17A or -17F as measured with the PCR array in systemic sclerosis (SSc) dermal fibroblasts

Project description:We examined the effect of IL-17 signaling pathway on extracellular matrix (ECM) expression and the involvement of IL-17 signaling pathway in pathogenesis of SSc. To identify differences in the expression pattern of ECM genes in IL-17A- or IL-17F-treated cells, we performed PCR array analysis, consisting of 84 ECM-related genes. Normal human dermal fibroblasts were cultured until they were confluent, and then stimulated with IL-17A or IL-17F for 12 hours, and total RNA was extracted. A mixture of equal amounts of mRNAs from three normal fibroblasts was prepared in the presence or absence of IL-17A or -17F, and mRNA expression profile was evaluated using PCR Array.

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Transcriptional profile comparing liver tissue in the absence/presence of LKB1 expression

Project description:The aim of this project is to determine the differential expression of genes between liver in the absence or presence of LKB1, leading to mitosis defects observed in absence of LKB1

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Identification BLM phosphosites in presence or absence of lambda phosphatase

Project description:Identification of BLM phosphosites in presence or absence of lambda phosphatase. DDA analysis of purified protein

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T cells activated in the presence or absence of TLR agonists

Project description:T cells activated in the presence or absence of TLR agonists

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Transcriptome of Helicobacter pylori in presence or absence of the cagPAI

Project description:To investigate the impact of the cagPAI on transcript regulation of H. pylori, RNA-seq was performed on bacterial samples with presence or absence of the cagPAI.

2023-04-21 | GSE227450 | GEO

T cells activated in the presence or absence of TLR agonists

Project description:We report the gene expression differences between OT-1 T cells activated alone or in the presence of TLR1/2 (Pam3CSK4) or TLR7 (Gardiquimod). By activating OT-1 splenocytes in the presence or absence of the TLR agonists, isolating RNA from purified CD8+ T cells we show that cells activated in the presence of either TLR1/2 or TLR7 agonists had similar transcriptional profiles demonstrating an increase in Th1 cytokine expression over cells that were activated alone. This study provides a key piece of information for those designing T cell mediated therapies.

2018-12-26 | GSE110144 | GEO

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