ABSTRACT: First detection in Helicobacter suis of a mutation conferring resistance to clarithromycin in Helicobacter pylori: case report and review of the literature
Project description:Analysis of Helicobacter pylori strain 26695 after 20 minutes of 0.25μg/ml Clarithromycin. Results provide insight into the mechanisms employed by the bacterium that help it adapt to Clarithromycin stress
Project description:Analysis of Helicobacter pylori strain 26695 after 20 minutes of 0.25μg/ml Clarithromycin. Results provide insight into the mechanisms employed by the bacterium that help it adapt to Clarithromycin stress In the study presented here, we compared the gene expression profile between H.pylori without CLA treatment and H.pylori treated with 0.25μg/ml Clarithromycin.
Project description:Rapidly and Reconditely Progressing Small Cell Lung Cancer with Invasive Pulmonary Aspergillosis: A Case Report and Literature Review
Project description:Thiele2005 - Genome-scale metabolic network
of Helicobacter pylori (iIT341)
This model is described in the article:
Expanded metabolic
reconstruction of Helicobacter pylori (iIT341 GSM/GPR): an in
silico genome-scale characterization of single- and
double-deletion mutants.
Thiele I, Vo TD, Price ND, Palsson
BØ.
J. Bacteriol. 2005 Aug; 187(16):
5818-5830
Abstract:
Helicobacter pylori is a human gastric pathogen infecting
almost half of the world population. Herein, we present an
updated version of the metabolic reconstruction of H. pylori
strain 26695 based on the revised genome annotation and new
experimental data. This reconstruction, iIT341 GSM/GPR,
represents a detailed review of the current literature about H.
pylori as it integrates biochemical and genomic data in a
comprehensive framework. In total, it accounts for 341
metabolic genes, 476 intracellular reactions, 78 exchange
reactions, and 485 metabolites. Novel features of iIT341
GSM/GPR include (i) gene-protein-reaction associations, (ii)
elementally and charge-balanced reactions, (iii) more accurate
descriptions of isoprenoid and lipopolysaccharide metabolism,
and (iv) quantitative assessments of the supporting data for
each reaction. This metabolic reconstruction was used to carry
out in silico deletion studies to identify essential and
conditionally essential genes in H. pylori. A total of 128
essential and 75 conditionally essential metabolic genes were
identified. Predicted growth phenotypes of single knockouts
were validated using published experimental data. In addition,
in silico double-deletion studies identified a total of 47
synthetic lethal mutants involving 67 different metabolic genes
in rich medium.
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Project description:The aim of this study is to identify alterations induced in gastric mucosa of mice exposed to Pteridium aquilinum and/or infected with Helicobacter pylori, in order to identify genes that are induced by bracken fern exerts exacerbating effects on gastric lesions associated to the infection. Six groups of C57Bl/6 mice were be used: 1) control, 2) infected Helicobacter pylori, 3) treated with Bracken fern extract orogastrically, 4) treated with Bracken fern extract in drinking water, 5) infected Helicobacter pylori + treated with Bracken fern extract orogastrically, 6) infected Helicobacter pylori + treated with Bracken fern extract in drinking water. The infection procedure was performed using an orogastric inoculation of H.pylori (strain SS1) twice in the first week. The RNA isolation was done in triplicate (3 mice per each condition). Further evaluation of morphological alterations on gastric mucosa, proliferative index and induction of DNA strand breaks will be performed in the mice stomach exposed to Pteridium aquilinum infected or not with Helicobacter pylori. Alterations of glycosylation in gastric tissues will also evaluated.
Project description:Primary objectives: Evaluation of the efficacy of eradication treatment of Helicobacter pylori. Proportion of eradication of H. pylori assessed at least 4 weeks after treatment is completed by the breath test, or alternatively, by urease test or histology when revision endoscopy is necessary
Primary endpoints: Efficacy in the eradication of antibiotic treatment in patients infected with Helicobacter pylori by breath test or histology, urease test or endoscopy if you need review
| 2530445 | ecrin-mdr-crc
Project description:Fungal keratitis caused by Colletotrichum dematium – case study and review of literature
Project description:Helicobacter pylori (H. pylori) is a human pathogen that infects almost half of the world’s population. Infection with H. pylori is frequently associated with chronic gastritis and can even lead to gastric and duodenal ulcers and gastric cancer. Although the persistent colonization of H. pylori and the development of H. pylori-associated gastritis remain poorly understood, it is believed that, in gastric mucosa, the modulated gastric epithelial cells (GECs) by H. pylori are key contributors. We used microarrays to detail the global programme of gene expression in Helicobacter pylori infected-gastric epithelial cell line AGS cells and identified up-regulated genes induced by Helicobacter pylori infection.
