Project description:We applied an iTRAQ-based quantitative proteomic approach to understand the differential proteome expression of marine diatom Skeletonema dohrnii grown in different temperature and silicate concentration.
Project description:Rising atmospheric CO2 concentrations are leading to ocean acidification, altering the inorganic carbon buffer system with consequences for marine organisms. Here we applied RNA-seq and iTRAQ quantification to investigate the potential impacts of ocean acidification on the temperate coastal marine diatom Skeletonema marinoi.
Project description:Purpose:Using Illumina high-throughout sequencing technology, the transcriptome and expression profiles of S. marinoi were sequenced and analyzed. Comprehensive and systematic study of transcriptomic characteristics, analysis of characteristics of Digital Gene Expression Profiling during different growth phase and deep exploration of molecular mechanism for response to the temperature and low silicate stress environment will provide a comprehensive insight into gene expression profiles and provides an important gene resource for future genetic and molecular studies of S. marinoi and other Skeletonema family diatom.
Project description:The experiment was designed to study the transcriptomic response of the centric diatom Skeletonema marinoi to the presence of cues from the grazer Calanus finmarchicus. Samples were collected in triplicate after 65 and 89 hours of exposure of diatom cells to the copepods. Control samples with no copepods were collected at the same time points.
