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Somatic reprogramming of pteropus bat cells provides new insights in bat antiviral response

ABSTRACT: Somatic reprogramming of pteropus bat cells provides new insights in bat antiviral response

PROVIDER: PRJNA555632 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9719900_1.fastq.gz	Fastqsanger.gz
	SRR9719900_2.fastq.gz	Fastqsanger.gz
	SRR9719901_1.fastq.gz	Fastqsanger.gz
	SRR9719901_2.fastq.gz	Fastqsanger.gz
	SRR9719902_1.fastq.gz	Fastqsanger.gz

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Somatic reprogramming of pteropus bat cells provides new insights in bat antiviral response

Project description:Bats are natural reservoirs for a large range of emerging viruses that cause lethal diseases in humans and domestic animals, but remain asymptomatic in bats. Understanding the host-pathogen interactions relies on the availability of relevant models including susceptible cells, derived from viral target tissues. To obtain bat cell types pertinent for the study of viral infection, we applied somatic reprogramming approach to Pteropus primary cells as initial substrates. Using the novel combination of three transcription factors: ESRRB, CDX2 and c-MYC, we generated reprogrammed cells exhibiting stem cells features.

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Project description:To examine innate immune signalling, IFN baseline and IRF-regulation, we profile Pteropus alecto bat kidney epithelial cells (PakiT03) with Deep NGS coverage. Clonal cell lines were also isolated with CrispR KO for IRF1, IRF3, IRF7, IFNAR2 or IFNAR2/IRF7 double KO.

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