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ABSTRACT:
PROVIDER: PRJNA557274 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Elsholtzia splendens rhizospheric microorganism Targeted loci environmental
| PRJNA306969 | ENA
Project description:Light spectrum quality is an important signal for plant growth and development. We aimed to analyze the effects of different light spectra on in vitro shoot development and proteomic and polyamine (PA) profiles in shoots of Cedrela fissilis. Cotyledonary and apical nodal segments were grown under different light emitting diode (LED) lamps and a fluorescent lamp. Shoots from cotyledonary nodal segments cultured with 6-benzyladenine (BA) grown under WmBdR LED increased their length, fresh and dry matter compared to shoots grown under fluorescent light. A non-redundant protein databank generated by transcriptome sequencing and de novo assembly of C. fissilis improved, and almost doubled, protein identification compared to a Citrus sinensis databank. Using the C. fissilis protein databank, a total of 616 proteins were identified, with 23 up- and 103 downaccumulated in shoots under WmBdR LED compared to fluorescent lamp. Differential accumulation of argininosuccinate synthase protein was associated with an increase in free-Put contents and, consequently, with higher shoot elongation under WmBdR LED. Furthermore, the proteins S-adenosylmethionine synthase, which is related to PA and ethylene biosynthesis, and 1-aminocyclopropane-1-carboxylate oxidase, related to ethylene biosynthesis, were unique in shoots grown under fluorescent lamp, showing lower elongation of shoots, possibly due to ethylene production. The downaccumulation of calreticulin, heat shock proteins, plastid-lipid-associated protein, ubiquitin-conjugating enzymes, and ultraviolet-B receptor UVR8 isoform X1 could be related to better shoot length under LED. This work provides important data related to the effects of light spectrum quality on in vitro morphogenesis via modulation of specific proteins and free-Put biosynthesis.
2020-09-04 | PXD018020 | Pride
Project description:We performed transcriptome assembly and gene expression analysis using short-read sequencing technology combined with a tag-based digital gene expression (DGE) system. The results generated a total number of 13,288,892 reads (accumulated length of 1,196,000,280 nt), 169,579 contings and 23,796 unigenes. Based on similarity search with known proteins, a total of 9,398 unigenes were identified with a cut-off E-value of 10-5. Assembled sequences were annotated with gene descriptions, such as gene ontology (GO) and clusters of orthologous group terms (COG). In addition, we obtained approximately 6 million raw tags and a larger number of genes at different fermentation stages (48 h, 100 h and 144 h). The related genes of growth characteristic and lipid biosynthesis were analyzed in detail. Some genes associated with the lipid biosynthesis were selected randomly to confirm digital gene expression (DGE) results by quantitative real-time PCR (qRT-PCR). The transcriptome improves our genetic understanding of Pythium splendens RBB-5 greatly and makes a large number of available gene sequences for further study. Notably, the transcriptome and DGE profiling data of Pythium splendens RBB-5 provide the comprehensive insight into gene expression profiles at different fermentation stages and lay a foundation for further study of optimizing lipid content and growth speed at the molecular level.
2014-12-30 | GSE43320 | GEO
Project description:microRNAs (miRNAs) play important roles in responses to abiotic stresses, including nutrition stress, by regulating target gene expression. Phosphate (Pi) is often lacking in natural and agro-climatic environments, and plants have developed strategies to cope with low Pi (LP) availability. However, the miRNA-mediated regulation of these adaptive responses and their underlying coordinating signals are still poorly understood in forestry trees such as Betula luminifera. Four small RNA (sRNA) libraries, a mixed degradome cDNA library, and four transcriptomic libraries of B. luminifera roots and shoots treated under LP and normal conditions (CK) were constructed and sequenced using next-generation deep sequencing. sRNA sequencing analyses indicated that 66 and 60 miRNAs were differentially expressed in roots and shoots, respectively, under LP conditions. A total of 109 and 112 miRNA–target pairs were further validated in the roots and shoots, respectively, using degradome sequencing, including several novel target genes that were cleaved by isomiRNAs with lengths of 18 or 19 nucleotides, which were only differentially expressed in roots. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of differential miRNA targets indicated that the “ascorbate and aldarate metabolism” pathway responded to LP, and “circadian rhythm – plant” was specifically enriched in shoots. A comprehensive B. luminifera transcriptome derived from its roots and shoots was constructed, and a total of 76,899 unigenes were generated. A comparison of transcriptome identified 8,095 and 5,584 differentially expressed genes in roots and shoots, respectively, under LP conditions. Integrated analysis uncovered 14 and 16 miRNA–target pairs that showed negatively correlated expression profiles in roots and shoots, respectively. Moreover, a putative model of miRNA–target interaction involved in plant responses to LP stress is proposed. These results suggest that comprehensive analyses of sRNAs, degradome, and transcriptome provide a useful platform for investigating LP stress in B. luminifera, and may provide new insights into the genetic engineering of high use efficiency of Pi in forestry trees.
2021-01-13 | GSE138335 | GEO
Project description:Calopteryx splendens genome sequencing
| PRJNA315816 | ENA
Project description:RNA was isolated from 23 old barley plants (shoots and roots), line Rolap. PARE libraries were constructed for both barley organs, followed by sequencing of NGS libraries.
2021-02-17 | GSE145423 | GEO