Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome upon Mof deletion and Etomoxir treatment in ground-state mouse (m) ESCs

ABSTRACT: Transcriptome upon Mof deletion and Etomoxir treatment in ground-state mouse (m) ESCs

PROVIDER: PRJNA564621 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10088749.fastq.gz	Fastqsanger.gz
	SRR10088750.fastq.gz	Fastqsanger.gz
	SRR10088751.fastq.gz	Fastqsanger.gz
	SRR10088752.fastq.gz	Fastqsanger.gz
	SRR10088753.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 10

Similar Datasets

Transcriptome upon Mof deletion and Etomoxir treatment in ground-state mouse (m) ESCs

Project description:This study describes the transcriptome profiling of: 1) Mof fl/fl mESCs cultured in 2i/LIF medium with Ethanol (WT) or 4-OHT (Mof-/-); 2) E14 mESCs cultured in 2i/LIF medium treated with H2O (control) or Etomoxir treatment (ETO, FAO inhibition) and ETO-released (Released).

2020-07-02 | GSE137138 | GEO

Derivation of ground-state female ESCs maintaining gamete-derived DNA methylation

Project description:Inhibitors of MEK1/2 and Gsk3b, known as '2i' culture conditions, enhance the derivation of embryonic stem cells (ESCs) and promote ground-state pluripotency in rodents 1,2. Here we show that the derivation of female mouse ESCs in the presence of 2i (2i-ESCs) results in a widespread loss of DNA methylation including a massive erasure of genomic imprints. Despite this global loss of DNA methylation, the early-passage 2i-ESCs efficiently differentiate into somatic cells and this process requires genome-wide de novo DNA methylation. However, the majority of imprinting control regions (ICRs) remain unmethylated in the 2i-ESC-derived differentiated cells. Consistently, 2i-ESCs exhibit impairment of autonomous embryonic and placental development by tetraploid embryo complementation and nuclear transplantation. We identified the derivation conditions of female ESCs that display 2i-ESC-like transcriptional signatures while preserving gamete-derived DNA methylation and autonomous developmental potential. Upon prolonged culture, however, female ESCs exhibit ICR demethylation, regardless of culture conditions. Our results provide insights into derivation of female ESCs reminiscent of ICM of preimplantation embryos. S-ESCs are ESCs established under serum-containing medium. 2i_S_ESCs are ESCs established in 2i-containing medium, followed by maintenance in serum-containing medium.

2017-07-27 | GSE84164 | GEO

Derivation of ground-state female ESCs maintaining gamete-derived DNA methylation [gene expression]

Project description:Preimplantation embryos undergo a transient wave of genome-wide demethylation with the exception of imprinted genes that are critical for fetal development. Here we show that the derivation of female mouse embryonic stem cells (ESCs) in the presence of inhibitors of MEK1/2 and Gsk3 (2i-ESCs), known as ‘2i’ or “ground-state” culture conditions, results in a widespread loss of DNA methylation including a massive erasure of genomic imprints. In this study, we analyzed global gene expression profile and global DNA methylation status in 2i-ESCs and 2i-ESCs derived differentiated cells. S-ESCs are ESCs established under serum-containing medium. 2i_S_ESCs are ESCs established in 2i-containing medium, followed by maintenance in serum-containing medium.

2017-07-27 | GSE82313 | GEO

Transcriptome of ESCs upon MLL1 inhibition/deletion

Project description:Transcriptome of ESCs upon MLL1 inhibition/deletion

| PRJNA420025 | ENA

Microarray analyses for wild type and Mof knockout murine ES cells.

Project description:We report the application of Affy based microarray technology for profiling of gene expression in mouse embryonic stem cells with or without histone acetytransferase MOF. We find that Mof regulates a wide range of genes in mouse ESCs including those important for ESC pluripotency and ESC differentiation. Mof deletion leads to similar number of genes that are up or down regulated. This study provides a framework for understanding the function of Mof in regulating ESC core transcription network.

2012-04-12 | GSE37109 | GEO

Genome wide mapping of histone acetyltransferase MOF binding in murine ES cells.

2012-04-11 | E-GEOD-37109 | biostudies-arrayexpress

Genome-wide expression patterns of microRNAs in ground state embryonic stem cells

Project description:We apply deep small-RNA sequencing technology for high-throughput profiling of microRNAs in ground state embryonic stem cells (ESCs). We provide global expression signatures of microRNAs in ESCs cultured under serum, 2i, and R2i conditions. We report that microRNAs are significantly differentially expressed when ESCs are cultured under different conditions, and that ground state pluripotency features a uniqure microRNA signature which is mainly encoded by microRNA-coding sequences within the developmentally important DLK1-Dio3 locus. Finally, we indicate that microRNA upregulated in ground state pluripotent cells (i.e. 2i/R2i) contribute to the maintenace of ground state pluripotency through stimulating self-renewal and inhibiting multi-lineague differentiation.

2017-09-21 | GSE87174 | GEO

Gene expression changes upon Mof knockout in MLL-AF9 transformed murine LSKs

Project description:We report Illumina next generation RNA sequencing (RNAseq) of MLL-AF9 in vitro transformed murine LSKs upon genetic deletion of Mof. These gene expression data illustrate that Mof regulates the expression of genes involved in DNA damage response and chromatin stability in MLL-AF9 transformed cells.

2017-08-30 | GSE80671 | GEO

TEAD2 mediates the ground-state pluripotency by chromatin looping

Project description:Mouse embryonic stem cells (mESCs) cultured in 2i (MEK and GSK3 kinase inhibitor)/LIF and serum/LIF that we called 2i-ESCs and serum-ESCs represent ground and confused pluripotent states, respectively. However, the transcription factors that regulate ground pluripotency through chromatin-associated characteristics are not yet fully understood. By mapping chromatin accessibility and transcription factor regulatory networks during the interconversion of 2i-ESCs and serum-ESCs, we have identified TEAD2 as highly enriched in 2i-specific peaks. While Tead2 knockout did not affect the pluripotency or differentiation ability of either 2i-ESCs or serum-ESCs, it did prevent the establishment of the 2i-specific state and the exit from the serum-specific state. TEAD2 binds to active regions in 2i-specific genes and activates their expression by regulating enhancer-promoter (EP) interactions during serum-to-2i transition. Remarkably, TEAD2-mediated EP interactions were independent of chromatin architecture proteins YY1 and CTCF, but instead appear to be facilitated by TEAD2 homodimer formation.

2024-03-03 | GSE226316 | GEO

Derivation of ground-state female ESCs maintaining gamete-derived DNA methylation

Project description:Derivation of ground-state female ESCs maintaining gamete-derived DNA methylation

| PRJNA328165 | ENA