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Expression data from isolated endothelial cells from glioblastoma and normal brain cultured as monolayer on gelatin

ABSTRACT: Expression data from isolated endothelial cells from glioblastoma and normal brain cultured as monolayer on gelatin

PROVIDER: PRJNA573743 | ENA |

REPOSITORIES: ENA

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Expression data from isolated endothelial cells from glioblastoma and normal brain cultured as monolayer on gelatin

Project description:We compared gene expressions between CD31-positive cells from glioblastoma (TECs) and endothelial cells from normal brain (NECs) In this dataset, expression data obtained from Affymetrix Human Genome U219 Array (HG-U219) are presented.

2022-05-18 | GSE137900 | GEO

Expression data from isolated endothelial cells from glioblastoma and normal brain cultured as monolayer on gelatin and on Matrigel

Project description:Expression data from isolated endothelial cells from glioblastoma and normal brain cultured as monolayer on gelatin and on Matrigel

| PRJNA573737 | ENA

Expression data from isolated endothelial cells from glioblastoma and normal brain cultured as monolayer on gelatin and on Matrigel

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-05-18 | GSE137902 | GEO

Expression data from isolated endothelial cells from glioblastoma and normal brain cultured on Matrigel

2022-05-18 | GSE137901 | GEO

RNA-seq analysis of mESCs cultured on either Laminin-511 or gelatin coated dishes in either Std or 2i medium

Project description:Some mouse embryonic stem cell (mESC) lines need to be maintained on feeder cells in gelatin/Std condition. To eliminate the need for feeder cells, we decided to maintain the C57BL6/J mESCs on dishes coated with Laminin-511 (LN511) enabling maintenance of mESCs without feeder cells in Std condition (Domogatskaya et al., 2008). To compare the transcriptomes of mESCs cultured on gelatin-coated dish and those cultured on Laminin-511 coated dish, we performed RNA-Seq analysis. We found that the transcriptomes of mESCs cultured on gelatin-coated dish and those cultured on LN511 coated dish showed no considerable difference in expression patterns.

2020-06-04 | GSE132591 | GEO

Expression data from isolated endothelial cells from glioblastoma and normal brain cultured on Matrigel

Project description:Expression data from isolated endothelial cells from glioblastoma and normal brain cultured on Matrigel

| PRJNA573744 | ENA

Transcriptome analysis of the differentiated C2C12 cells in 3 different substrate, Tissue culture plate, Methacarylated gelatin and 15% Methacarylated gelatin mixed with 1% PSS (poly sodium 4-styrenesulfonate)

Project description:Purpose : To determine transcriptome profile of the diffferentiated C2C12 cells in 3 different cell culture substrate, Tissue culture plate, Methacarylated gelatin and 15% Methacarylated gelatin mixed with 1% PSS (poly sodium 4-styrenesulfonate) Method : RNA smples prepared from differentiated C2C12 cells in 3 different cell culture substrate, Tissue culture plate, Methacarylated gelatin and 15% Methacarylated gelatin mixed with 1% PSS (poly sodium 4-styrenesulfonate) Results : Paired-ends 101bp reads, we mapped about 72 million reads - 145 million reads per sample to the mouse genome (build mm10) and assembled with cufflinks program (value : FPKM)

2021-01-26 | GSE165480 | GEO

Perivascular signals alter global genomic profile of glioblastoma and response to temozolomide in a gelatin hydrogel

Project description:Perivascular signals alter global genomic profile of glioblastoma and response to temozolomide in a gelatin hydrogel

| PRJNA436235 | ENA

Transcriptomes of high-grade serous ovarian cancer cell lines cultured in monolayer and in spheroids.

Project description:HGSOC cancer cell lines OvCAR3 and G164 were cultured in monolayer and spheroids and subjected to RNA-sequencing.

2024-05-02 | GSE235099 | GEO

Polyvinyl alcohol/gelatin hydrogels regulate cell adhesion and chromatin accessibility

Project description:Cell adhesion is known to heavily influence cell migration and wound healing. A number of cell adhesion molecules and their roles has been extensively studied. However, the changes of global chromatin accessibility and gene expressions correlated with cell adhesion is still unclear. Here we prepared a series of PVA/Gelatin hydrogels to handily regulate cell adhesion by adjusting gelatin concentration and found that cell adhesion area and the ratio of non-circular cells were increased with the increasement of gelatin concentration while cell circularity was decreased. Our results showed that widespread chromatin accessibility was increased with increasing expression level of histone deacetylase 11 (Hdc11) under poor cell adhesion. Meanwhile, one branch of MAPK pathway - ERK 1/2 which involved in mechanotransduction signaling was also found in the enrichment analysis of downregulated genes. This work provided a handy method to regulate cell adhesion and these findings deepen our understanding of the cell adhesion process and its associated chromatin accessibility.

2022-08-11 | GSE190091 | GEO

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