Project description:The leaf transcriptome of the nickel hyperaccumulator Psychotria gabriellae (Rubiaceae) endemic from New Caledonia was compared to the closely related non-accumulator Psychotria semperflorens, living in sympatry with P. gabriellae in rain forest on serpentine soil, to identity differentially expressed genes potentially involved in Ni hyperaccumulation.
Project description:Rauwolfia vomitoria extract is an herbal preparation of the root bark of a plant in the tropical forests of Africa, which could inhibit Benign prostatic hyperplasia (BPH). Characterizing the molecular alterations of BPH1 and WPMY-1 cells treated with RWF is important for understanding the molecular mechanisms of RWF inhibiting BPH. We used microarrays to detail the RNA expression.
Project description:A comparision of soil microbial functional genes of three types of subtropical broad-leaved forests Microbial functional structure was significantly different among SBFs (P < 0.05). Compared to the DBF and the EBF, the MBF had higher alpha-diversity of functional genes but lower beta-diversity, and showed more complex functional gene networks.
Project description:Characterization of the genomic distribution of RAIN in thyroid cancer. RAIN was pulled-down using two sets of 3’ BiotinTEG probes (EVEN and ODD). Subsequently, the DNA bound by RAIN was sequenced to assess its biding genome wide.
Project description:Ganoderma colossum (Fr.) Baker, a white rot fungus distributed in tropical and subtropical forests, is a rare Ganoderma mushroom belonging to the family Ganodermataceae. In Asia, the fruiting body of G. colossum has been used as tonics or as folk medicine for a variety of illnesses, including malignant diseases. Colossolactone H (colo H) as a new compound was isolated and studied for its anticancer mechanism in human lung cancer H1650 cells. We used microarrays to analyze the gene expression changes upon colo H treatment that helped underlying the molecular mechanism of colo H in H1650 lung cancer cells. The H1650 cells treated with colo H for 12 and 24 h for RNA extraction and hybridization on Affymetrix microarrays.
Project description:MicroRNAs (miRNAs) are non-coding, short, single-stranded RNAs with essential roles in gene regulation in various organisms including higher plants. In contrast to the vast information on miRNAs from many economically important plants, almost nothing has been reported on the identification or analysis of miRNAs from rubber tree (Hevea brasiliensis L.), the most important natural rubber-producing crop. To identify miRNAs and their target genes in rubber tree, high throughput sequencing combined with a computational approach was performed. Four small RNA libraries were constructed for deep sequencing from mature and young leaves of two rubber tree clones, PB 260 and PB 217, which provide high and low latex yield, respectively. 237 miRNAs belonging to 37 known miRNA families were identified, and northern hybridization validated miRNA expression and revealed developmental stage-dependent and clone-specific expression for some miRNAs. We took advantage of the newly released rubber tree genome assembly as well as the genomic databases from leafy spurge and cassava, two species related to rubber tree, and predicted 15 novel miRNAs.
2012-04-03 | GSE35254 | GEO
Project description:Rhizosphere Microbial Diversity of Northern Tropical Seasonal Rain Forest
Project description:Ganoderma colossum (Fr.) Baker, a white rot fungus distributed in tropical and subtropical forests, is a rare Ganoderma mushroom belonging to the family Ganodermataceae. In Asia, the fruiting body of G. colossum has been used as tonics or as folk medicine for a variety of illnesses, including malignant diseases. Colossolactone H (colo H) as a new compound was isolated and studied for its anticancer mechanism in human lung cancer H1650 cells. We used microarrays to analyze the gene expression changes upon colo H treatment that helped underlying the molecular mechanism of colo H in H1650 lung cancer cells.
Project description:Drivers of tropical soil invertebrate community composition and richness across tropical secondary forests using DNA metasystematics
