Tubifex tubifex
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ABSTRACT:
PROVIDER: PRJNA584690 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Tubifex tubifex EST - Tutu_0003 transcriptome
| PRJNA584691 | ENA
Project description:Tubifex tubifex EST - Tutu_0001 transcriptome
| PRJNA584689 | ENA
Project description:Tubifex tubifex breed:Annelida Genome sequencing
| PRJNA725344 | ENA
Project description:Tubifex tubifex breed:Annelida Genome sequencing
| PRJNA725065 | ENA
Project description:BackgroundTubifex tubifex is a widespread annelid characterized by considerable variability in its taxonomic characteristics and by a mixed reproductive strategy, with both parthenogenesis and biparental reproduction. In a molecular phylogenetic analysis, we detected substantial genetic variability among sympatric Tubifex spp. from the Lambro River (Milano, Italy), which we suggested comprise several cryptic species. To gain insights into the evolutionary events that generated this differentiation, we performed a cytogenetic analysis in parallel with a molecular assay. Approximately 80 cocoons of T. tubifex and T. blanchardi were collected and dissected. For each cocoon, we sequenced a fragment of the 16S rRNA from half of the sibling embryos and karyotyped the other half. To generate a robust phylogeny enabling the reconstruction of the evolutionary processes shaping the diversity of these sympatric lineages, we complemented our original 16S rRNA gene sequences with additional COI sequences.ResultsThe chromosome number distribution was consistent with the presence of at least six sympatric euploid chromosome complements (one diploid, one triploid, three tetraploids and one hexaploid), as confirmed by a FISH assay performed with an homologous 18S rDNA probe. All the worms with 2n = 50 chromosomes belonged to an already identified sibling species of T. tubifex, T. blanchardi. The six euploid sets were coherently arranged in the phylogeny, with each lineage grouping specimens with the same chromosome complement.ConclusionsThese results are compatible with the hypothesis that multiple polyploidization events, possibly enhanced by parthenogenesis, may have driven the evolution of the T. tubifex species complex.
| S-EPMC4021366 | biostudies-literature
Project description:Global transcriptome patterns were determined in 2-week-old RD26-IOE seedlings after 2h (one biological replicate) or 5h (two biological replicates) estradiol (EST) or ethanol (mock) treatment. EST-induced expression of RD26.
2019-01-01 | GSE100654 | GEO
Project description:Dufulin is a highly effective antiviral pesticide used in plants. In this study, a seven-day experiment was conducted to evaluate the effects of Dufulin at five different concentrations (1 × 10-4, 1 × 10-3, 1 × 10-2, 0.1, and 1 mg/L) on Tubifex. LC-MS-based metabolome analysis detected a total of 5356 features in positive and 9110 features in negative, of which 41 showed significant changes and were identified as differential metabolites. Four metabolic pathways were selected for further study. Detailed analysis revealed that Dufulin exposure affected the urea cycle of Tubifex, probably via argininosuccinate lyase (ASL) inhibition. It also affected the fatty acid metabolism, leading to changes in the concentration of free fatty acids in Tubifex. Furthermore, the changes in metabolites after exposure to Dufulin at 1 × 10-2 mg/L were different from those at the other concentrations.
| S-EPMC8231163 | biostudies-literature
Project description:Global transcriptome patterns were determined in XVE-14 and wild-type seedlings induced for 45 min b-estradiol in order to identify the genes early regulated by EBE transcription factor. We used microarrays to identify genes differentially expressed in EST-inducible EBE over-expression line #14 compared to wild-type plants, 45 min after 2µM EST induction. Three independent biological replications were performed.
2013-03-30 | GSE27855 | GEO
Project description:Global transcriptome patterns were determined in MYB112-IOE seedlings induced for 3 h and 5 h with b-estradiol in order to identify the genes early regulated by MYB112 transcription factor. We used microarrays to identify genes differentially expressed in EST-inducible MYB112 over-expression line #1, 3 h and 5 h after 10µM EST induction, compared to mock treated plants. Two independent biological replications were performed for 5 h timepoint and one replicate for 3 h timepoint.
2015-11-03 | GSE36721 | GEO