Project description:The immune microenvironment of hepatocellular carcinoma (HCC) is poorly characterized. Combining two single-cell RNA sequencing technologies, we produced transcriptomes of CD45+ immune cells for HCC patients from five immune-relevant sites: tumor, adjacent liver, hepatic lymph node (LN), blood, and ascites.A cluster of LAMP3+ dendritic cells (DCs) appeared to be the mature form of conventional DCs and possessed the potential to migrate from tumors to LNs. LAMP3+ DCs also expressed diverse immune-relevant ligands and exhibited potential to regulate multiple subtypes of lymphocytes. Of the macrophages in tumors that exhibited distinct transcriptional states, tumor-associated macrophages (TAMs) were associated with poor prognosis, and we established the inflammatory role of SLC40A1 and GPNMB in these cells. Further, myeloid and lymphoid cells in ascites were predominantly linked to tumor and blood origins, respectively. The dynamic properties of diverse CD45+ cell types revealed by this study add new dimensions to the immune landscape of HCC.
Project description:Mongolia has the highest reported incidence of (and mortality from) hepatocellular carcinoma (HCC) in the world. This is the first molecular study aiming to characterize the genomic landscape of Mongolian HCC.
Project description:As immunotherapy plays an increasingly important role in the treatment of hepatocellular carcinoma, the immune infiltration heterogeneity of the tumor provides an important reference for immunotherapy decisions. In this study, a mouse hepatocellular carcinoma model was established, and the intra-tumor and peritumor tissues were sampled and analyzed by RNA sequencing, respectively, to explore the heterogeneity of immune-related gene expression in intra- and peritumor regions.