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Cellular trajectory analysis links tissue maturation to cellular specialization in the plant meristem [protophloem sieve element cells]

ABSTRACT: Cellular trajectory analysis links tissue maturation to cellular specialization in the plant meristem [protophloem sieve element cells]

PROVIDER: PRJNA596429 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10733464.fastq.gz	Fastqsanger.gz
	SRR10733465.fastq.gz	Fastqsanger.gz
	SRR10733466.fastq.gz	Fastqsanger.gz
	SRR10733467.fastq.gz	Fastqsanger.gz
	SRR10733468.fastq.gz	Fastqsanger.gz

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Cellular trajectory analysis links tissue maturation to cellular specialization in the plant meristem

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Cellular trajectory analysis links tissue maturation to cellular specialization in the plant meristem [protophloem sieve element cells]

Project description:The mechanisms that allow cells in the plant meristem to coordinate tissue-wide maturation gradients with specialized cell networks are critical for indeterminate growth. Here, we combine long-term imaging, single-cell transcriptomics, genetic analysis, and network modeling to reconstruct the differentiation trajectory of the 20 protophloem cells in the Arabidopsis root. We found that lineage bifurcation and cellular specification are mediated near the stem cell niche by PEAR regulators. However, many PEAR downstream effects are repressed by a broad gradient of PLETHORA transcription factors, which directly inhibit PEARs’ own direct target APL until dissipating seven cells from the stem cell niche. Modeling and molecular epistasis suggest a so-called “seesaw” cascade of sharp transitions in which transcription factors regulating consecutive steps mutually inhibit each other’s targets, ending in programmed enucleation. This analysis provides a mechanistic understanding of how morphogen-like maturation gradients interface with cell-type specific transcriptional regulators to stage cellular differentiation

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Project description:Meristem maintenance, achieved through the highly conserved CLAVATA-WUSCHEL (CLV-WUS) regulatory circuit, is fundamental in balancing stem cell proliferation with cellular differentiation. Disruptions to meristem homeostasis can alter meristem size, leading to enlarged organs. Cotton, the world’s most important fiber crop, shows inherent variation in fruit size. Leveraging meristem regulation could benefit cotton agriculture and increase yields of high-quality fibers. Using virus-based gene manipulation in cotton, we altered expression of genes functioning in the CLV-WUS circuit to perturb meristem regulation and increase fruit size. Targeted alteration of individual components of the CLV-WUS circuit only modestly fasciated flowers and fruits. Unexpectedly, controlled expression of meristem regulator SELF-PRUNING (SP) dramatically increased the impacts of altered CLV-WUS expression on flower and fruit fasciation. Meristem transcriptomics show SP and genes of the CLV-WUS circuit are expressed independently from each other, suggesting these gene products are not acting in the same path. Virus-induced silencing of GhSP facilitated delivery of other signals to the meristem to dramatically alter organ specification. SELF-PRUNING has a role in cotton meristem homeostasis, and changes in GhSP expression increased access of virus-based signals to the meristem.

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