RNA Sequencing - Zebrafish FASD Model
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ABSTRACT:
PROVIDER: PRJNA596534 | ENA |
REPOSITORIES: ENA
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Project description:Purpose: The transcriptional alterations underlying physological changes in fetal alcohol spectrum disorder are largely unidentified. Here we perform RNA sequencing on control and embryonic alcohol exposed (EAE/FAE) zebrafish at larval (13 dpf) and adult stages in order to identify significant transcriptional alterations under normal and HFHC diet conditions.
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2013-04-30 | E-GEOD-45706 | biostudies-arrayexpress
Project description:The present study was conducted in the frame of the EU-funded Graphene Flagship project. We previously evaluated the impact of graphene oxide (GO) on the gut microbiome in adult zebrafish by performing 16S rRNA gene sequencing in wild-type versus AhR-deficient zebrafish. Here, we performed single-cell RNA-sequencing (10x Genomics) on whole (dissociated) germ-free (GF) zebrafish embryos exposed at 5 dpf to GO plus the microbial metabolite butyrate to gain insight into the impact on specific cell populations in GF zebrafish.
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Project description:Purpose: study of function of ATG5 protein on zebrafish larvae development Methods: Using morpholino oligos for atg5 gene knock down and overexpression, injected into one- to four-cell-stage embryos,extracted total RNA, then sending to RNA sequencing. Results: we compared to Wildtype, 5MO and atg5 mRNA groups, we analyza autophagy related gene mRNA expression, there are obvious different. Conclusion: our study showed the function of autophagy related gene 5 on zebrafish larvae, it has an important significant for understanding the affection of autophagy on embryo development
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