Project description:The ascidia Halocynthia roretzi (Phylum: Chordata, Subphylum: Tunicata, Class: Ascidiacea) have been used as model species in development biology for over a century. It offers attractive experimental features, including a compact genome, invariant embryonic cell lineages, small embryonic cell number, and translucent embryos, which allow the description of developmental processes with a cellular level of resolution. Staged RNA-seq data has been already deposited in DDBJ (accessiton number DRA005714 and DRA005711). In the present study, we sequenced genome of H. roretzi of a southwestern Japanese population using RNA-Seq data. These RNA-Seq data covers expressions in 8 cell and 110 cell embryos of Animal(A), Vegetal(V) and whole-embryo regions. These genome assembly, transcript assembly, and transcript models are incorporated into the ANISEED (https://www.aniseed.cnrs.fr/) for genome browsing and blast searches. The genome and transcriptome resources will be useful datasets for developmental biology, evolutionary biology and molecular ecology using this model organism.
Project description:The primary objective of this prospective observational study is to characterize the gut and oral microbiome as well as the whole blood transcriptome in gastrointestinal cancer patients and correlate these findings with cancer type, treatment efficacy and toxicity. Participants will be recruited from existing clinical sites only, no additional clinical sites are needed.
