Project description:The goat of this project is to explore lncRNA5251 regulation spermatogenesis. We tried to search the mechanism oflncRNA5251 regulation spermatogenesis. RNA-seq of F1 mouse testis samples from different groups: 5NC, lncRNA5251 overexpression, 3NC, lncRNA5251 knockdown. The testes of mouse were transfected with virus with 5NC, lncRNA5251 overexpression, 3NC, lncRNA5251 knocdown letivirus.
Project description:The goat of this project is to explore lncRNA8276 regulation spermatogenesis. We tried to search the mechanism oflncRNA8276 regulation spermatogenesis. RNA-seq of F1 mouse testis samples from different groups: 5NC, lncRNA8276 overexpression, 3NC, lncRNA8276 knockdown. The testes of mouse were transfected with virus with 5NC, lncRNA8276 overexpression, 3NC, lncRNA8276 knocdown letivirus.
Project description:Embryonic exposure to the endocrine disruptor vinclozolin during gonadal sex determination appears to promote an epigenetic reprogramming of the male germ line that is associated with transgenerational adult-onset disease states. Transgenerational effects on the embryonic day 16 (E16) testis demonstrated reproducible changes in the testis transcriptome for multiple generations (F1-F3). The expression of 196 genes was found to be influenced, with the majority of gene expression being decreased or silenced. Dramatic changes in the gene expression of methyltransferases during gonadal sex determination were observed in the F1 and F2 vinclozolin generation (E16) embryonic testis, but the majority returned to control-generation levels by the F3 generation. The most dramatic effects were on the germ-line-associated Dnmt3A and Dnmt3L isoforms. Observations demonstrate that an embryonic exposure to vinclozolin appears to promote an epigenetic reprogramming of the male germ line that correlates with transgenerational alterations in the testis transcriptome in subsequent generations. Experiment Overall Design: E16 Testis RNA samples from F1, F2, F3 generation control groups are compared to F1, F2, F3 generation vinclozolin treated groups
Project description:The goat of this project is to explore lncRNA5251 regulation spermatogenesis. We tried to search the mechanism oflncRNA5251 regulation spermatogenesis. RNA-seq of F0 mouse testis samples from different groups: 5NC, lncRNA5251 overexpression, 3NC, lncRNA5251 knockdown. The testes of mouse were transfected with virus with 5NC, lncRNA5251 overexpression, 3NC, lncRNA5251 knocdown letivirus.
Project description:The goat of this project is to explore lncRNA8276 regulation spermatogenesis. We tried to search the mechanism oflncRNA8276 regulation spermatogenesis. RNA-seq of F0 mouse testis samples from different groups: 5NC, lncRNA8276 overexpression, 3NC, lncRNA8276 knockdown. The testes of mouse were transfected with virus with 5NC, lncRNA8276 overexpression, 3NC, lncRNA8276 knocdown letivirus.