Project description:To determine how SAHA-PIP G can trigger the expression of the mesendoderm-related genes in mouse ES cells, chromatin immunoprecipitation (ChIP-seq) analysis was carried out with an antibody against the histone H3 lysine 4 trimethylation (H3K4me3) using EBs harvested on day 0
Project description:Endothelium in embryonic hematopoietic tissues generates hematopoietic stem/progenitor cells; however, it is unknown how its unique potential is specified. We show that transcription factor Scl/Tal1 is essential for both establishing the hematopoietic transcriptional program in hemogenic endothelium and preventing its misspecification to a cardiomyogenic fate. Scl-/- embryos activated a cardiac transcriptional program in yolk sac endothelium, leading to the emergence of CD31+Pdgfrα+ cardiogenic precursors that generated spontaneously beating cardiomyocytes. Ectopic cardiogenesis was also observed in Scl-/- hearts, where the disorganized endocardium precociously differentiated into cardiomyocytes. Induction of mosaic deletion of Scl in Sclfl/flRosa26Cre-ERT2 embryos revealed a cell-intrinsic, temporal requirement for Scl to prevent cardiomyogenesis from endothelium. Scl-/- endothelium also upregulated the expression of Wnt antagonists, which promoted rapid cardiomyocyte differentiation of ectopic cardiogenic cells. These results reveal unexpected plasticity in embryonic endothelium such that loss of a single master regulator can induce ectopic cardiomyogenesis from endothelial cells.
Project description:To identify salt-resistant related genes by histone deacetylase inhibitor Ky-2, expression profiles between plants treated with or without Ky-2 under salt stress were analyzed using the custom microarray (GPL20781).
Project description:To identify salt-resistant related genes by histone deacetylase inhibitor Ky-2, expression profiles between plants treated with or without Ky-2 under salt stress were analyzed using the custom microarray (GPL20781). Wild-type plants (Arabidopsis thaliana ecotype Columbia) were grown on 1/2 MS 0.1 % Agar medium for four days. Then, the plants were administrated to Ky-2 for 24 hours and then treated 100 mM Nacl for two hours. Expression profiles were analyzed using the custom array (GPL20781).