Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterization of the Wheat-Psathyrostachys huashania 2Ns/2D Substitution Line H139: A Novel Germplasm with Enhanced Resistance to Wheat Take-all

ABSTRACT: Characterization of the Wheat-Psathyrostachys huashania 2Ns/2D Substitution Line H139: A Novel Germplasm with Enhanced Resistance to Wheat Take-all

PROVIDER: PRJNA599164 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Characterization of the Wheat-Psathyrostachys huashania 2Ns/2D Substitution Line H139: A Novel Germplasm with Enhanced Resistance to Wheat Take-all

Project description:Take-all is a devastating soil-borne disease that affects wheat production. The continuous generation of disease-resistance germplasm is an important aspect of the management of this pathogen. In this study, we characterized the wheat-Psathyrostachys huashania Keng-derived progeny H139 that exhibits significantly improved resistance to wheat take-all disease compared with its susceptible parent 7182. GISH) and mc-FISH analyses revealed that H139 is a stable wheat-P. huashania disomic substitution line lacking wheat chromosome 2D.EST-STS marker and Wheat Axiom 660K Genotyping Array analysis further revealed that H139 was a novel wheat-P. huashania 2Ns/2D substitution line, and that the P. huashania 2Ns chromosome shares high sequence similarity to wheat chromosome 2D. These results indicate that H139, with its enhanced wheat take-all disease resistance and desirable agronomic traits, provides valuable genetic resources for wheat chromosome engineering breeding.

2020-01-07 | GSE143188 | GEO

Cotton Fiber Cells: Germplasm Line MD 52ne vs. Germplasm Line MD 90ne

2009-01-14 | GSE14388 | GEO

Cotton Fiber Cells: Germplasm Line MD 52ne vs. Germplasm Line MD 90ne

Project description:Transcriptional profiling of cotton fiber cells from two cotton germplasm lines, MD 52ne and MD 90ne. Comparison of fiber cell transcription profiles is between the two germplasm lines and over a developmental time-course from 8 to 24 days post anthesis in four day intervals. Cotton plants grown in 3-4 row plots of approximately 300-400 individual plants. Bulked fiber samples from multiple plants per each plot represented a biological replication. There were 3-4 spatially distinct plots per cotton germplasm line. Loop microarray hybridization experimental design. Biological replicates: 2 for each germplasm line at each time-point. Technical replicates: 2 for each germplasm line at each time-point (dye-swap).

2010-05-07 | E-GEOD-14388 | biostudies-arrayexpress

Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [alien substitution lines]

Project description:Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [alien substitution lines]

2023-06-30 | MSV000092320 | MassIVE

Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [alien substitution lines]

Project description:Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [alien substitution lines] experiment 1

2023-06-30 | MSV000092323 | MassIVE

Characterization of the Wheat-Psathyrostachys huashania Keng 2Ns/2D Substitution Line H139: A Novel Germplasm With Enhanced Resistance to Wheat Take-All.

Project description:Take-all is a devastating soil-borne disease that affects wheat production. The continuous generation of disease-resistance germplasm is an important aspect of the management of this pathogen. In this study, we characterized the wheat-Psathyrostachys huashania Keng (P. huashania)-derived progeny H139 that exhibits significantly improved resistance to wheat take-all disease compared with its susceptible parent 7182. Sequential genomic in situ hybridization (GISH) and multicolor fluorescence in situ hybridization (mc-FISH) analyses revealed that H139 is a stable wheat-P. huashania disomic substitution line lacking wheat chromosome 2D. Expressed sequence tag-sequence tagged site (EST-STS) marker and Wheat Axiom 660K Genotyping Array analysis further revealed that H139 was a novel wheat-P. huashania 2Ns/2D substitution line. In addition, the H139 line was shown to be cytologically stable with a dwarf phenotype and increased spikelet number. These results indicate that H139, with its enhanced wheat take-all disease resistance and desirable agronomic traits, provides valuable genetic resources for wheat chromosome engineering breeding.

| S-EPMC7077511 | biostudies-literature

GNPS - Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [alien substitution lines]

Project description:Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [alien substitution lines]

2023-06-30 | MSV000092320 | GNPS

GNPS - Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [alien substitution lines]

Project description:Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [alien substitution lines] experiment 1

2023-06-30 | MSV000092323 | GNPS

Transcription profiling of wheat variety Chinese Spring and two derived chromosome 5A substitution lines treated with cold stress

Project description:Chromosome 5A of wheat is a major regulator of freezing tolerance. It harbours gene loci controlling freezing tolerance (Fr-A1 and Fr-A2) and vernalization requirement (Vrn-A1). The number of cold-responsive genes and their functional classification was studied by cDNA macroarray-based transcript profiling in the moderately freezing-sensitive wheat (Triticum aestivum L.) variety Chinese Spring and two derived chromosome 5A substitution lines exhibiting lower or higher levels of freezing tolerance, respectively. During 21-days of cold acclimation at 2 °C the transcript level changed significantly for 681 (6.6%) out of 10,297 studied unigenes. The freezing-tolerant substitution line exhibited about 1.5-fold higher number of differentially expressed genes compared to the sensitive one. Transcript levels of several genes were altered by cold treatment only in one of the three genotypes. For 78 genes regulation by factors hosted on chromosome 5A was determined and postulated. These genes encoded proteins involved in transcriptional regulation, defence processes and carbohydrate metabolism. Three of the chromosome 5A-affected genes, having especially strong and rapid cold-induced changes of transcript level, namely Tacr7 (Triticum aesticum cold-responsive gene 7 homolog), Cab (Ca2+-binding), and Dem (deficient embryo and meristems) were genetically mapped and characterized in further detail. Only the gene Cab was located on chromosome 5A, whereas Tacr7 and Dem resided on other chromosomes. Although chromosome 5A-affected genes are also found to be localised on the same chromosome, the present findings indicate that chromosome 5A is strongly involved in trans-regulation of genes potentially due to the presence of regulatory loci, like Fr-A2, which is harboring numerous CBF transcription factors.

2009-06-22 | E-IPKG-10 | biostudies-arrayexpress

Radish germplasm resequencing

Project description:Resequencing data for radish germplasm

| PRJEB44749 | ENA