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Determining the role of serum progesterone monitoring by comparing endometrial transcriptomics in women with low progesterone versus women with normal progesterone during the receptive phase of the menstrual cycle

ABSTRACT: Determining the role of serum progesterone monitoring by comparing endometrial transcriptomics in women with low progesterone versus women with normal progesterone during the receptive phase of the menstrual cycle

PROVIDER: PRJNA601132 | ENA |

REPOSITORIES: ENA

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Endometrial methylome of healthy women during the transition from pre-receptive to receptive phase

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Determining the role of serum progesterone monitoring by comparing endometrial transcriptomics in women with low progesterone versus women with normal progesterone during the receptive phase of the menstrual cycle

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The Extracellular Vesicles Proteome of Endometrial Cells Simulating the Receptive Menstrual Phase Differs from That of Endometrial Cells Simulating the Non-Receptive Menstrual Phase

Project description:Successful embryo implantation into a receptive endometrium requires mutual endometrial-embryo communication. Recently, the function of extracellular vehicles (EVs) in cell-to-cell interaction in embryo-maternal interactions has been investigated. We explored isolated endometrial derived EVs, using RL95-2 cells as a model of a receptive endometrium, influenced by menstrual cycle hormones estrogen (E2; proliferative phase) progesterone (P4; secretory phase) and estrogen plus progesterone (E2P4; the receptive phase). EV sized particles were isolated by differential centrifugation and size exclusion chromatography. Nanoparticle tracking analysis was used to examine the different concentration and size of particles and EV proteomic analysis per-formed using shotgun label-free mass spectrometry. Our results showed that although endome-trial derived EVs were secreted in numbers independent of hormonal stimulation, EVs sizes were statistically modified by it. Proteomics analysis showed that hormone treatment changes affect the endometrial EVs proteome, with proteins enhanced within the EV E2P4 group shown to be in-volved in different processes such as embryo implantation, endometrial receptivity, and embryo development, supporting the concept of a communication system between the embryo and the maternal endometrium via EVs.

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Transcriptomics of receptive endometrium in women with sonographic features of adenomyosis

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