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Xenopsylla cheopis strain:RML

ABSTRACT: Transcriptomic Profiling of the Digestive Tract of the Rat Flea, Xenopsylla cheopis

PROVIDER: PRJNA601490 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10902900_1.fastq.gz	Fastqsanger.gz
	SRR10902900_2.fastq.gz	Fastqsanger.gz
	SRR10902901_1.fastq.gz	Fastqsanger.gz
	SRR10902901_2.fastq.gz	Fastqsanger.gz
	SRR10902902_1.fastq.gz	Fastqsanger.gz

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A substrate-like thrombin inhibitor from the saliva of the flea Xenopsylla cheopis is resistant to cleavage by the enzyme

Project description:XC-43 is a small peptide identified in the sialome of the flea Xenopsylla cheopis and act as a fast, tight-binding inhibitor of thrombin with a dissociation constant of less than 10 pM. The crystal structure of XC-43 in complex with thrombin shows that despite its substrate-like binding mode, XC-43 is not detectably cleaved by thrombin and that it interacts with the thrombin surface from the enzyme catalytic site through the fibrinogen-binding exosite I. The low rate of hydrolysis is verified in solution experiments with XC-43 which show the substrate to be largely intact after two hours of incubation with thrombin at 37°C. The potential of XC-43 as an anticoagulant is suggested by increased arterial occlusion time, tail bleeding time, and blood coagulation parameters in rat models of thrombosis.

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Project description:. Here we provide a deeper insight into Xenopsylla cheopis salivary glands contents pairing transcriptome and proteomic approaches. Sequencing of 99 pairs of salivary glands from adult females X. cheopis yielded a total of 7,432 coding sequences functionally classified into 25 classes, in which the secreted class was found to be the most abundant one. The translated transcripts also served as a reference database for the proteomic study, which identified peptides from 610 different proteins.

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