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Coordination of germ-layer lineage choice by TET1 during primed pluripotency (ATAC-seq)

ABSTRACT: Coordination of germ-layer lineage choice by TET1 during primed pluripotency (ATAC-seq)

PROVIDER: PRJNA605182 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11035112.fastq.gz	Fastqsanger.gz
	SRR11035113.fastq.gz	Fastqsanger.gz
	SRR11035114.fastq.gz	Fastqsanger.gz
	SRR11035115.fastq.gz	Fastqsanger.gz
	SRR11035116.fastq.gz	Fastqsanger.gz

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Coordination of germ-layer lineage choice by TET1 during primed pluripotency

Project description:Coordination of germ-layer lineage choice by TET1 during primed pluripotency

| PRJNA605177 | ENA

Coordination of germ-layer lineage choice by TET1 during primed pluripotency (ChIP-seq)

Project description:Coordination of germ-layer lineage choice by TET1 during primed pluripotency (ChIP-seq)

| PRJNA605181 | ENA

Coordination of germ-layer lineage choice by TET1 during primed pluripotency (ChIP-seq)

Project description:Gastrulation in the early post-implantation stage mammalian embryo begins when epiblast cells ingress to form the primitive streak or develop as the embryonic ectoderm. The DNA dioxygenase Tet1 is highly expressed in the epiblast and yet continues to regulate lineage specification during gastrulation when its expression is diminished. Here, we show how Tet1 plays a pivotal role upstream of germ layer lineage bifurcation. During the transition from naive pluripotency to lineage priming, a global reconfiguration re-distributes Tet1 from Oct4 co-bound promoters to distal regulatory elements at lineage differentiation genes which are distinct from high-affinity sites engaged by Oct4. An altered chromatin landscape in Tet1-deficient primed epiblast-like cells is associated with enhanced Oct4 expression and binding to Nodal and Wnt target genes, resulting in collaborative signals that enhance mesendodermal and inhibit neuroectodermal gene expression during lineage segregation. A permissive role for Tet1 in neural fate induction involves Zic2-dependent engagement at neural target genes at lineage priming, is dependent on the signaling environment during gastrulation and impacts neural tube closure at post-gastrulation. Our findings provide mechanistic information for epigenetic integration of pluripotency and signal-induced differentiation cues.

2020-03-02 | GSE144868 | GEO

Coordination of germ-layer lineage choice by TET1 during primed pluripotency (ATAC-seq)

2020-03-02 | GSE144867 | GEO

Coordination of germ layer lineage choice by TET1 during primed pluripotency.

Project description:Gastrulation in the early postimplantation stage mammalian embryo begins when epiblast cells ingress to form the primitive streak or develop as the embryonic ectoderm. The DNA dioxygenase Tet1 is highly expressed in the epiblast and yet continues to regulate lineage specification during gastrulation when its expression is diminished. Here, we show how Tet1 plays a pivotal role upstream of germ layer lineage bifurcation. During the transition from naive pluripotency to lineage priming, a global reconfiguration redistributes Tet1 from Oct4-cobound promoters to distal regulatory elements at lineage differentiation genes, which are distinct from high-affinity sites engaged by Oct4. An altered chromatin landscape in Tet1-deficient primed epiblast-like cells is associated with enhanced Oct4 expression and binding to Nodal and Wnt target genes, resulting in collaborative signals that enhance mesendodermal and inhibit neuroectodermal gene expression during lineage segregation. A permissive role for Tet1 in neural fate induction involves Zic2-dependent engagement at neural target genes at lineage priming, is dependent on the signaling environment during gastrulation, and impacts neural tube closure after gastrulation. Our findings provide mechanistic information for epigenetic integration of pluripotency and signal-induced differentiation cues.

| S-EPMC7111260 | biostudies-literature

Coordination of germ-layer lineage choice by TET1 during primed pluripotency

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-03-02 | GSE144869 | GEO

The catalytic activity of TET1 is required for human germ cell fate choice [bACE-Seq]

Project description:Primordial germ cell (PGC) is the foundation of the germline which become gametes and transmit genetic and epigenetic information to the next generation. The segregation of germline and soma is critical yet well-characterized in human during embryonic development due to ethical consideration. Here we discover that human TET1 (Ten-eleven translocation 1) acts as a safety check of germline cell fate. Using CRISPR/Cas9 to knock-out TET1 catalytic domain, we demonstrate extremely low hPGCLC (hPGC-like cell) competency of undifferentiated pluripotent stem cell lines. We plot whole-genome 5’hydroxymethylation (5hmC) profiles to elucidate global as well as locus-specific 5hmC enrichment in hPGCLCs, indicating the DNA oxygenase activity of TET1 is active during hPGCLC differentiation.

2023-09-12 | GSE218910 | GEO